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	A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica
	Wang, GG; Li, YH; Xia, P; Duan, DL
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2005
卷号	17期号:1页码:75-79
关键词	Brown Alga Dna Extraction Laminaria Japonica Polysaccharides Sporophyte
ISSN号	0921-8971
DOI	10.1007/s10811-005-5557-9
文献子类	Article
英文摘要	A simple method was developed for extracting DNA from brown algae Laminaria japonica, which possess large amounts of acidic polysaccharides. Firstly, the sporophyte were washed by eliminating polysaccaride buffer to remove the polysaccharides and then ground in liquid nitrogen. Secondly, the powders were treated with lysing buffer. Thirdly, KAc was used to eliminate the remaining acidic polysaccharides. The extracted DNA was purified using a chloroform-isoamyl alcohol ( 24: 1 v/v), and precipitated in cold isopropanol. The yield was from 18.7 to 37.5 mu g g(-1) (wet weight) and the purity of total DNA was determined spectrophotometrically as the ratio of A(260)/A(280), which was about 1.7 - 1.9. The extracted DNA was of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion. This method is a reproducible, simple, and rapid technique for routine DNA extraction from sporophyte in Laminaria japonica. Furthermore, the low cost of this method makes it attractive for large-scale studies.; A simple method was developed for extracting DNA from brown algae Laminaria japonica, which possess large amounts of acidic polysaccharides. Firstly, the sporophyte were washed by eliminating polysaccaride buffer to remove the polysaccharides and then ground in liquid nitrogen. Secondly, the powders were treated with lysing buffer. Thirdly, KAc was used to eliminate the remaining acidic polysaccharides. The extracted DNA was purified using a chloroform-isoamyl alcohol ( 24: 1 v/v), and precipitated in cold isopropanol. The yield was from 18.7 to 37.5 mu g g(-1) (wet weight) and the purity of total DNA was determined spectrophotometrically as the ratio of A(260)/A(280), which was about 1.7 - 1.9. The extracted DNA was of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion. This method is a reproducible, simple, and rapid technique for routine DNA extraction from sporophyte in Laminaria japonica. Furthermore, the low cost of this method makes it attractive for large-scale studies.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000229904700012
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3135]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Wang, GG,Li, YH,Xia, P,et al. A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2005,17(1):75-79.
APA	Wang, GG,Li, YH,Xia, P,&Duan, DL.(2005).A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica.JOURNAL OF APPLIED PHYCOLOGY,17(1),75-79.
MLA	Wang, GG,et al."A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica".JOURNAL OF APPLIED PHYCOLOGY 17.1(2005):75-79.

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