Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers
	Sun, JW ; Jin, DM ; Zhou, CJ ; Yang, QK ; Weng, ML ; Duan, DL ; Xu, P; Ma, JH ; Wang, B
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2005-09-01
卷号	23 期号:3 页码:251-262
关键词	Amplified Fragment Length Polymorphism Dna Fingerprinting Porphyra Line Sequence Characterized Amplified Region Sequence Tagged Site (Sts) Specific Molecular Marker
ISSN号	0735-9640
文献子类	Article
英文摘要	Twenty-seven Porphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results, computerized AFLP DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique AFLP,fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germplasm identification-AFLP) was designed for identification of the 27 Porphyra lines. In addition, 21 specific AFLP markers from 15 Porphyra lines were identified; 6 AFLP markers from 4 Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification, and resource protection of the Porphyra lines.; Twenty-seven Porphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results, computerized AFLP DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique AFLP,fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germplasm identification-AFLP) was designed for identification of the 27 Porphyra lines. In addition, 21 specific AFLP markers from 15 Porphyra lines were identified; 6 AFLP markers from 4 Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification, and resource protection of the Porphyra lines.
学科主题	Biochemical Research Methods ; Plant Sciences
语种	英语
WOS记录号	WOS:000235577400005
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3127]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Laiyang Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Jiangsu Inst Oceanol & Hydrol, Nantong 226007, Peoples R China 5.Shanghai Fisheries Univ, Shanghai 200090, Peoples R China
推荐引用方式 GB/T 7714	Sun, JW,Jin, DM,Zhou, CJ,et al. Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers[J]. PLANT MOLECULAR BIOLOGY REPORTER,2005,23(3):251-262.
APA	Sun, JW.,Jin, DM.,Zhou, CJ.,Yang, QK.,Weng, ML.,...&Wang, B.(2005).Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers.PLANT MOLECULAR BIOLOGY REPORTER,23(3),251-262.
MLA	Sun, JW,et al."Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers".PLANT MOLECULAR BIOLOGY REPORTER 23.3(2005):251-262.