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	Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor
	Deng, Xiangyuan1,2; Qin, Song1; Zhang, Qun1,2; Jiang, Peng1; Cui, Yulin1,2; Li, Xiaokun3
刊名	PLANT CELL TISSUE AND ORGAN CULTURE
	2009-06-01
卷号	97期号:3页码:253-261
关键词	Microprojectile Bombardment Bubble-column Bioreactor Laminaria Japonica Gametophytes Human Acidic Fibroblast Growth Factor
ISSN号	0167-6857
DOI	10.1007/s11240-009-9523-0
文献子类	Article
英文摘要	A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.; A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000265942400004
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2897]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Jinan Univ, Biopharmaceut Res & Dev Ctr, Guangzhou 510632, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Deng, Xiangyuan,Qin, Song,Zhang, Qun,et al. Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2009,97(3):253-261.
APA	Deng, Xiangyuan,Qin, Song,Zhang, Qun,Jiang, Peng,Cui, Yulin,&Li, Xiaokun.(2009).Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor.PLANT CELL TISSUE AND ORGAN CULTURE,97(3),253-261.
MLA	Deng, Xiangyuan,et al."Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor".PLANT CELL TISSUE AND ORGAN CULTURE 97.3(2009):253-261.

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