Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation | |
Wang, Jinfeng1,2; Jiang, Peng1; Cui, Yulin1,2; Guan, Xiangyu1; Qin, Song1; Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China | |
刊名 | PHYCOLOGIA |
2010-07-01 | |
卷号 | 49期号:4页码:355-360 |
关键词 | Egfp Genetic Transformation Lacz Porphyra Haitanensis Sv40 Promoter |
ISSN号 | 0031-8884 |
DOI | 10.2216/09-91.1 |
文献子类 | Article |
英文摘要 | Genetic transformation by electroporation of protoplasts is a standard procedure for many plants. However, for the genus Porphyra, the method is not effective because of low viability of protoplasts and is a time-consuming and expensive procedure. Based on the life history of Porphyra, a spore-targeted strategy of genetic transformation was developed, that is, using fresh conchospores of Porphyra haitanensis Chang & Zheng transformed by agitation with glass beads. A SV40 promoter-driven lacZ reporter gene was expressed in conchospores 48 h after the agitation. More transformants were obtained by increasing the agitation time from 10 to 25 s. The maximum number of transformants was more than six out of 1 million agitated conchospores. Transfer of a SV40 promoter-driven egfp gene into conchospores resulted in significant green GFP fluorescence. The expression of lacZ and egfp revealed that this strategy of spore-targeted transformation using glass bead agitation is feasible in P. haitanensis and that the SV40 promoter is effective for monitoring expression of foreign genes in this red algal species.; Genetic transformation by electroporation of protoplasts is a standard procedure for many plants. However, for the genus Porphyra, the method is not effective because of low viability of protoplasts and is a time-consuming and expensive procedure. Based on the life history of Porphyra, a spore-targeted strategy of genetic transformation was developed, that is, using fresh conchospores of Porphyra haitanensis Chang & Zheng transformed by agitation with glass beads. A SV40 promoter-driven lacZ reporter gene was expressed in conchospores 48 h after the agitation. More transformants were obtained by increasing the agitation time from 10 to 25 s. The maximum number of transformants was more than six out of 1 million agitated conchospores. Transfer of a SV40 promoter-driven egfp gene into conchospores resulted in significant green GFP fluorescence. The expression of lacZ and egfp revealed that this strategy of spore-targeted transformation using glass bead agitation is feasible in P. haitanensis and that the SV40 promoter is effective for monitoring expression of foreign genes in this red algal species. |
学科主题 | Plant Sciences ; Marine & Freshwater Biology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000280232300006 |
公开日期 | 2010-11-18 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/1684] |
专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
通讯作者 | Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Jinfeng,Jiang, Peng,Cui, Yulin,et al. Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation[J]. PHYCOLOGIA,2010,49(4):355-360. |
APA | Wang, Jinfeng,Jiang, Peng,Cui, Yulin,Guan, Xiangyu,Qin, Song,&Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.(2010).Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation.PHYCOLOGIA,49(4),355-360. |
MLA | Wang, Jinfeng,et al."Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation".PHYCOLOGIA 49.4(2010):355-360. |
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