Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca)

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	Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca)
	Wang, YP ; Xu, Z ; Guo, XM ; Guo, XM, Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, 6959 Miller Ave, Port Norris, NJ 08349 USA
刊名	BIOLOGICAL BULLETIN
	2004-02-01
卷号	206 期号:1 页码:46-54
关键词	In-situ Hybridization Nucleolus Organizer Regions Mitochondrial-dna Sequences Ribosomal-rna Gene Gigas Thunberg Constitutive Heterochromatin Rivularis Gould Oyster Karyotype Location
ISSN号	0006-3185
文献子类	Article
英文摘要	Karyotype and chromosomal location of the major ribosomal RNA genes (rDNA) were studied using fluorescence in situ hybridization (FISH) in five species of Crassostrea: three Asian-Pacific species (C. gigas, C. plicatula, and C. ariakensis) and two Atlantic species (C. virginica and C. rhizophorae). FISH probes were made by PCR amplification of the intergenic transcribed spacer between the 18S and 5.8S rRNA genes, and labeled with digoxigenin-11-dUTP. All five species had a haploid number of 10 chromosomes. The Atlantic species had 1-2 submetacentric chromosomes, while the three Pacific species had none. FISH with metaphase chromosomes detected a single telomeric locus for rDNA in all five species without any variation. In all three Pacific species, rDNA was located on the long arm of Chromosome 10 (10q)-the smallest chromosome. In the two Atlantic species, rDNA was located on the short arm of Chromosome 2 (2p)-the second longest chromosome. A review of other studies reveals the same distribution of NOR sites (putative rDNA loci) in three other species: on 10q in C. sikamea and C. angulata from the Pacific Ocean and on 2p in C. gasar from the western Atlantic. All data support the conclusion that differences in size and shape of the rDNA-bearing chromosome represent a major divide between Asian-Pacific and Atlantic species of Crassostrea. This finding suggests that chromosomal divergence can occur under seemingly conserved karyotypes and may play a role in reproductive isolation and speciation.; Karyotype and chromosomal location of the major ribosomal RNA genes (rDNA) were studied using fluorescence in situ hybridization (FISH) in five species of Crassostrea: three Asian-Pacific species (C. gigas, C. plicatula, and C. ariakensis) and two Atlantic species (C. virginica and C. rhizophorae). FISH probes were made by PCR amplification of the intergenic transcribed spacer between the 18S and 5.8S rRNA genes, and labeled with digoxigenin-11-dUTP. All five species had a haploid number of 10 chromosomes. The Atlantic species had 1-2 submetacentric chromosomes, while the three Pacific species had none. FISH with metaphase chromosomes detected a single telomeric locus for rDNA in all five species without any variation. In all three Pacific species, rDNA was located on the long arm of Chromosome 10 (10q)-the smallest chromosome. In the two Atlantic species, rDNA was located on the short arm of Chromosome 2 (2p)-the second longest chromosome. A review of other studies reveals the same distribution of NOR sites (putative rDNA loci) in three other species: on 10q in C. sikamea and C. angulata from the Pacific Ocean and on 2p in C. gasar from the western Atlantic. All data support the conclusion that differences in size and shape of the rDNA-bearing chromosome represent a major divide between Asian-Pacific and Atlantic species of Crassostrea. This finding suggests that chromosomal divergence can occur under seemingly conserved karyotypes and may play a role in reproductive isolation and speciation.
学科主题	Biology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000189314600006
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1584]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Guo, XM, Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, 6959 Miller Ave, Port Norris, NJ 08349 USA
作者单位	1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, YP,Xu, Z,Guo, XM,et al. Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca)[J]. BIOLOGICAL BULLETIN,2004,206(1):46-54.
APA	Wang, YP,Xu, Z,Guo, XM,&Guo, XM, Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, 6959 Miller Ave, Port Norris, NJ 08349 USA.(2004).Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca).BIOLOGICAL BULLETIN,206(1),46-54.
MLA	Wang, YP,et al."Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca)".BIOLOGICAL BULLETIN 206.1(2004):46-54.