Isolation and biosynthesis of labdanmycins: four new labdane diterpenes from endophytic Streptomyces

	Isolation and biosynthesis of labdanmycins: four new labdane diterpenes from endophytic Streptomyces
	Xiong, Zi-Jun; Huang, Jianping; Yan, Yijun; Wang, Li; Wang, Zhiyan; Yang, Jing; Luo, Jianying; Li, Jie; Huang, Sheng-Xiong
刊名	ORGANIC CHEMISTRY FRONTIERS
	2018
卷号	5 期号:8 页码:1272-1279
ISSN号	2052-4129
英文摘要	Chemical investigation of Streptomyces sp. KIB 015, an endophyte from the plant Panax notoginseng, led to the discovery of two novel labdane diterpenes, labdanmycins A (1) and B (2). In order to reveal the biosynthesis of labdanmycins, the genome of S. sp. KIB 015 was sequenced and a lab gene cluster encoding GGPP synthase, class II cyclase, class I cyclase, and cytochrome P450s was confirmed to be involved in labdanmycin biosynthesis by heterologous expression and gene inactivation experiments. Biogenetic precursor raimonol (3) was isolated from the gene labE inactivation mutant strain S. sp. KIB 015.labE, which suggested that P450 enzyme LabE is responsible for oxidizing the C-20 methyl group of 3 to an aldehyde or carbonyl group to afford 1 and 2. Additional two new labdane diterpenes (4 and 5) were also isolated from the mutant S. sp. KIB 015 Delta labE. The chemical structures of 1-5 were elucidated on the basis of extensive NMR and MS spectroscopic analyses.
语种	英语
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/64747]
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Xiong, Zi-Jun,Huang, Jianping,Yan, Yijun,et al. Isolation and biosynthesis of labdanmycins: four new labdane diterpenes from endophytic Streptomyces[J]. ORGANIC CHEMISTRY FRONTIERS,2018,5(8):1272-1279.
APA	Xiong, Zi-Jun.,Huang, Jianping.,Yan, Yijun.,Wang, Li.,Wang, Zhiyan.,...&Huang, Sheng-Xiong.(2018).Isolation and biosynthesis of labdanmycins: four new labdane diterpenes from endophytic Streptomyces.ORGANIC CHEMISTRY FRONTIERS,5(8),1272-1279.
MLA	Xiong, Zi-Jun,et al."Isolation and biosynthesis of labdanmycins: four new labdane diterpenes from endophytic Streptomyces".ORGANIC CHEMISTRY FRONTIERS 5.8(2018):1272-1279.