The Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian

doi:10.1128/AEM.01965-17

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	The Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian
	Danchin, Antoine(Hop La Pitie Salpetriere, Inst Cardiometab & Nutr, Paris, France); Zhu, Fang-Chao2 ; Zhang, Pei-Wei 2; He, Li-Sheng2 ; Huang, Jiao-Mei2 ; Wang, Yong(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China); Danchin, Antoine 1; Wang, Yong2
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2018
卷号	84 期号:1
关键词	Symbiosis Hadal Crispr Genome Reduction Spiroplasma
DOI	10.1128/AEM.01965-17
产权排序	第1完成单位 ; 第2完成单位
文献子类	Article
英文摘要	Protective symbiosis has been reported in many organisms, but the molecular mechanisms of the mutualistic interactions between the symbionts and their hosts are unclear. Here, we sequenced the 424-kbp genome of "Candidatus Spiroplasma holothuricola," which dominated the hindgut microbiome of a sea cucumber, a major scavenger captured in the Mariana Trench (6,140 m depth). Phylogenetic relationships indicated that the dominant bacterium in the hindgut was derived from a basal group of Spiroplasma species. In this organism, the genes responsible for the biosynthesis of amino acids, glycolysis, and sugar transporters were lost, strongly suggesting endosymbiosis. The highly decayed genome consists of two chromosomes and harbors genes coding for proteolysis, microbial toxin, restrictionmethylation systems, and clustered regularly interspaced short palindromic repeats (CRISPRs), composed of three cas genes and 76 CRISPR spacers. The holothurian host is probably protected against invading viruses from sediments by the CRISPRs/Cas and restriction systems of the endosymbiotic spiroplasma. The protective endosymbiosis indicates the important ecological role of the ancient Spiroplasma symbiont in the maintenance of hadal ecosystems.
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WOS关键词	PSEUDOMONAS-AERUGINOSA ; MODIFICATION SYSTEMS ; INSECT ENDOSYMBIONT ; GUT MICROBIOTA ; SEQUENCE ; BACTERIA ; METAGENOMES ; WOLBACHIA ; EVOLUTION ; ARGININE
语种	英语
WOS记录号	WOS:000418039700026
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/5203]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组深海科学研究部_深海生物学研究室
通讯作者	Danchin, Antoine(Hop La Pitie Salpetriere, Inst Cardiometab & Nutr, Paris, France); Wang, Yong(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China); Danchin, Antoine; Wang, Yong
作者单位	1.Hop La Pitie Salpetriere, Inst Cardiometab & Nutr, Paris, France 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Danchin, Antoine,Zhu, Fang-Chao,Zhang, Pei-Wei,et al. The Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(1).
APA	Danchin, Antoine.,Zhu, Fang-Chao.,Zhang, Pei-Wei.,He, Li-Sheng.,Huang, Jiao-Mei.,...&Wang, Yong.(2018).The Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(1).
MLA	Danchin, Antoine,et al."The Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.1(2018).