Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas

doi:10.1111/mec.14149

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas
	Sun, Jin 4; Wong, Yue Him 4; Li, Runsheng 2; Tabata, Harry G.4; Bartlett, Douglas H.3; He, Li-Sheng1 ; Xu, Ting 2; Qiu, Jian-Wen 2; Zhang, Weipeng 4; Qian, Pei-Yuan 4
刊名	MOLECULAR ECOLOGY
	2017-07-01
卷号	26 期号:14 页码:3732-3743
关键词	Challenger Deep Deep Sea Hydrostatic Pressure Mariana Trench Positive Selection
DOI	10.1111/mec.14149
产权排序	第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位
文献子类	Article
英文摘要	The Challenger Deep in the Mariana Trench is the deepest point in the oceans of our planet. Understanding how animals adapt to this harsh environment characterized by high hydrostatic pressure, food limitation, dark and cold is of great scientific interest. Of the animals dwelling in the Challenger Deep, amphipods have been captured using baited traps. In this study, we sequenced the transcriptome of the amphipod Hirondellea gigas collected at a depth of 10,929 m from the East Pond of the Challenger Deep. Assembly of these sequences resulted in 133,041 contigs and 22,046 translated proteins. Functional annotation of these contigs was made using the GO and KEGG databases. Comparison of these translated proteins with those of four shallow-water amphipods revealed 10,731 gene families, of which 5659 were single-copy orthologs. Base substitution analysis on these single-copy orthologs showed that 62 genes are positively selected in H. gigas, including genes related to beta-alanine biosynthesis, energy metabolism and genetic information processing. For multiple-copy orthologous genes, gene family expansion analysis revealed that cold-inducible proteins (i.e., transcription factors II A and transcription elongation factor 1) as well as zinc finger domains are expanded in H. gigas. Overall, our results indicate that genetic adaptation to the hadal environment by H. gigas may be mediated by both gene family expansion and amino acid substitutions of specific proteins.
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WOS关键词	HELICASE-II GU ; HYDROSTATIC-PRESSURE ; POSITIVE SELECTION ; RNA HELICASE ; MAXIMUM-LIKELIHOOD ; HIGH-THROUGHPUT ; MARIANA TRENCH ; PROTEIN ; GENOME ; ANNOTATION
语种	英语
WOS记录号	WOS:000404618000013
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/3962]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者	Qian, Pei-Yuan; Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China)
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China 2.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China 3.Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA 4.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jin,Wong, Yue Him,Li, Runsheng,et al. Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas[J]. MOLECULAR ECOLOGY,2017,26(14):3732-3743.
APA	Sun, Jin.,Wong, Yue Him.,Li, Runsheng.,Tabata, Harry G..,Bartlett, Douglas H..,...&Lan, Yi.(2017).Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas.MOLECULAR ECOLOGY,26(14),3732-3743.
MLA	Sun, Jin,et al."Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas".MOLECULAR ECOLOGY 26.14(2017):3732-3743.