Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing

doi:10.3389/fmicb.2015.01172

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物分子生态学研究组

	Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing
	Jing, Hongmei3 ; Xia, Xiaomin 2; Liu, Hongbin 2; Zhou, Zhi 1; Wu, Chen 1; Nagarajan, Sanjay 1
刊名	FRONTIERS IN MICROBIOLOGY
	2015-10-21
卷号	6
关键词	Diazotrophs Nifh Pyrosequencing Mangrove
DOI	10.3389/fmicb.2015.01172
文献子类	Article
英文摘要	Diazotrophs in the mangrove rhizosphere play a major role in providing new nitrogen to the mangrove ecosystem and their composition and activity are strongly influenced by anthropogenic activity and ecological conditions. In this study, the diversity of the diazotroph communities in the rhizosphere sediment of five tropical mangrove sites with different levels of pollution along the north and south coastline of Singapore were studied by pyrosequencing of the nifH gene. Bioinformatics analysis revealed that in all the studied locations, the diazotroph communities comprised mainly of members of the diazotrophic cluster I and cluster III. The detected cluster III diazotrophs, which were composed entirely of sulfate reducing bacteria, were more abundant in the less polluted locations. The metabolic capacities of these diazotrophs indicate the potential for bioremediation and resiliency of the ecosystem to anthropogenic impact. In heavily polluted locations, the diazotrophic community structures were markedly different and the diversity of species was significantly reduced when compared with those in a pristine location. This, together with the increased abundance of Marinobacterium, which is a bioindicator of pollution, suggests that anthropogenic activity has a negative impact on the genetic diversity of diazotrophs in the mangrove rhizosphere.
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WOS关键词	SULFATE-REDUCING BACTERIA ; NITROGEN-FIXING BACTERIA ; METHANOSARCINA-BARKERI 227 ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC DIVERSITY ; DINITROGEN FIXATION ; GENE DIVERSITY ; RIVER ESTUARY ; HEAVY-METALS ; SEDIMENTS
语种	英语
WOS记录号	WOS:000363980100001
内容类型	期刊论文
版本	出版稿
源URL	[http://124.16.218.2:8080/handle/183446/889]
专题	深海科学研究部_深海生物学研究室_海洋微生物分子生态学研究组
作者单位	1.Natl Univ Singapore, Fac Engn, Dept Civil & Environm Engn, Singapore 117548, Singapore 2.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Jing, Hongmei,Xia, Xiaomin,Liu, Hongbin,et al. Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing[J]. FRONTIERS IN MICROBIOLOGY,2015,6.
APA	Jing, Hongmei,Xia, Xiaomin,Liu, Hongbin,Zhou, Zhi,Wu, Chen,&Nagarajan, Sanjay.(2015).Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing.FRONTIERS IN MICROBIOLOGY,6.
MLA	Jing, Hongmei,et al."Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing".FRONTIERS IN MICROBIOLOGY 6(2015).