Visualization of Alzheimer's Disease Related alpha-/beta-/gamma-Secretase Ternary Complex by Bimolecular Fluorescence Complementation Based Fluorescence Resonance Energy Transfer | |
Wang, Xin1; Pei, Gang1,2,3 | |
刊名 | FRONTIERS IN MOLECULAR NEUROSCIENCE |
2018 | |
卷号 | 11期号:1页码:431 |
关键词 | Amyloid Precursor Protein Beta-secretase App Site Cleavage Adam10 Bace1 Localization Dimerization Endocytosis |
ISSN号 | 1662-5099 |
DOI | 10.3389/fnmol.2018.00431 |
文献子类 | Article |
英文摘要 | The competitive ectodomain shedding of amyloid-beta) precursor protein (APP) by alpha-secretase and beta-secretase, and the subsequent regulated intramembrane proteolysis by gamma-secretase are the key processes in amyloid-beta peptides (A beta) generation. Previous studies indicate that secretases form binary complex and the interactions between secretases take part in substrates processing. However, whether alpha-, beta- and gamma-secretase could form ternary complex remains to be explored. Here, we adopted bimolecular fluorescence complementation in combination with fluorescence resonance energy transfer (BiFC-FRET) to visualize the formation of triple secretase complex. We show that the interaction between alpha-secretase ADAM10 and beta-secretase BACE1 could be monitored by BiFC assay and the binding of APP to alpha-/beta-secretase binary complex was revealed by BiFC-FRET. Further, we observed that gamma-secretase interacts with alpha-/beta-secretase binary complex, providing evidence that alpha-, beta- and gamma-secretase might form a ternary complex. Thus our study extends the interplay among Alzheimer's disease (AD) related alpha-/beta-/gamma-secretase. |
WOS研究方向 | Neurosciences |
语种 | 英语 |
WOS记录号 | WOS:000451483200002 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3512] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Pei, Gang |
作者单位 | 1.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Chinese Acad Sci, Shanghai, Peoples R China; 2.Tongji Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 3.Tongji Univ, Collaborat Innovat Ctr Brain Sci, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xin,Pei, Gang. Visualization of Alzheimer's Disease Related alpha-/beta-/gamma-Secretase Ternary Complex by Bimolecular Fluorescence Complementation Based Fluorescence Resonance Energy Transfer[J]. FRONTIERS IN MOLECULAR NEUROSCIENCE,2018,11(1):431. |
APA | Wang, Xin,&Pei, Gang.(2018).Visualization of Alzheimer's Disease Related alpha-/beta-/gamma-Secretase Ternary Complex by Bimolecular Fluorescence Complementation Based Fluorescence Resonance Energy Transfer.FRONTIERS IN MOLECULAR NEUROSCIENCE,11(1),431. |
MLA | Wang, Xin,et al."Visualization of Alzheimer's Disease Related alpha-/beta-/gamma-Secretase Ternary Complex by Bimolecular Fluorescence Complementation Based Fluorescence Resonance Energy Transfer".FRONTIERS IN MOLECULAR NEUROSCIENCE 11.1(2018):431. |
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