Structural insights into the sequence-specific recognition of Piwi by Drosophila Papi | |
Zhang, Yuhan1,2,3; Li, Ronghong1,2; Wu, Ligang1,2; Huang, Ying1,2,3; Wu, Ping3,6; Peng, Chao3,6; Liu, Weiwei4; Gu, Jiaqi4,5; Yu, Yang4,6; Ma, Jinbiao5 | |
刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
2018 | |
卷号 | 115期号:13页码:3374-3379 |
关键词 | Tudor-domain Protein Pirna 3' Ends Arginine Methylation Interacting Rna Royal Family Biogenesis Pathway Nuage Ago3 Transcription |
ISSN号 | 0027-8424 |
DOI | 10.1073/pnas.1717116115 |
文献子类 | Article |
英文摘要 | The Tudor domain-containing (Tdrd) family proteins play a critical role in transposon silencing in animal gonads by recognizing the symmetrically dimethylated arginine (sDMA) on the (G/A)R motif of the N-terminal of PIWI family proteins via the eTud domains. Papi, also known as "Tdrd2," is involved in Zucchini-mediated PIWI-interacting RNA (piRNA) 3'-end maturation. Intriguingly, a recent study showed that, in papi mutant flies, only Piwi-bound piRNAs increased in length, and not Ago3-bound or Aub-bound piRNAs. However, the molecular and structural basis of the Papi-Piwi complex is still not fully understood, which limitsmechanistic understanding of the function of Papi in piRNA biogenesis. In the present study, we determined the crystal structures of Papi-eTud in the apo form and in complex with a peptide containing unmethylated or dimethylated R10 residues. Structural and biochemical analysis showed that the Papi interaction region on the Drosophila Piwi contains an RGRRR motif (R7-R11) distinct from the consensus (G/A) R motif recognized by canonical eTud. Mass spectrometry results indicated that Piwi is the major binding partner of Papi in vivo. The papi mutant flies suffered from both fertility and transposon-silencing defects, supporting the important role conferred to Papi in piRNA 3' processing through direct interaction with Piwi proteins. |
WOS研究方向 | Multidisciplinary Sciences |
语种 | 英语 |
WOS记录号 | WOS:000428382400057 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3481] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Huang, Ying |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl, State Key Lab Mol Biol,CAS Ctr Excellence Mol Cel, Shanghai 200031, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China; 4.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China; 5.Fudan Univ, State Key Lab Genet Engn, Collaborat Innovat Ctr Genet & Dev, Dept Biochem,Sch Life Sci, Shanghai 200438, Peoples R China; 6.Natl Facil Prot Sci Shanghai, Zhangjiang Lab, Shanghai 201210, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Yuhan,Li, Ronghong,Wu, Ligang,et al. Structural insights into the sequence-specific recognition of Piwi by Drosophila Papi[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(13):3374-3379. |
APA | Zhang, Yuhan.,Li, Ronghong.,Wu, Ligang.,Huang, Ying.,Wu, Ping.,...&Ma, Jinbiao.(2018).Structural insights into the sequence-specific recognition of Piwi by Drosophila Papi.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(13),3374-3379. |
MLA | Zhang, Yuhan,et al."Structural insights into the sequence-specific recognition of Piwi by Drosophila Papi".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.13(2018):3374-3379. |
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