Structural and biochemical insights into small RNA 3 ' end trimming by Arabidopsis SDN1 | |
Chen, Jiayi1; Gu, Jiaqi1; Ruan, Wenjie1; Zhang, Lu1; Ma, Jinbiao1; Liu, Li2; You, Chenjiang2; Chen, Xuemei2; Gan, Jianhua3; Cao, Chunyang4 | |
刊名 | NATURE COMMUNICATIONS |
2018 | |
卷号 | 9期号:1页码:3585 |
关键词 | Microrna Biogenesis Target Rnas Protein Nmr Drosophila Exonuclease Nibbler Degradation Argonaute1 Elegans Sirnas |
ISSN号 | 2041-1723 |
DOI | 10.1038/s41467-018-05942-7 |
文献子类 | Article |
英文摘要 | A family of DEDDh 3'-> 5' exonucleases known as Small RNA Degrading Nucleases (SDNs) initiates the turnover of ARGONAUTE1 (AGO1)-bound microRNAs in Arabidopsis by trimming their 3' ends. Here, we report the crystal structure of Arabidopsis SDN1 (residues 2-300) in complex with a 9 nucleotide single-stranded RNA substrate, revealing that the DEDDh domain forms rigid interactions with the N-terminal domain and binds 4 nucleotides from the 3' end of the RNA via its catalytic pocket. Structural and biochemical results suggest that the SDN1 C-terminal domain adopts an RNA Recognition Motif (RRM) fold and is critical for substrate binding and enzymatic processivity of SDN1. In addition, SDN1 interacts with the AGO1 PAZ domain in an RNA-independent manner in vitro, enabling it to act on AGO1-bound microRNAs. These extensive structural and biochemical studies may shed light on a common 3' end trimming mechanism for 3'-> 5' exonucleases in the metabolism of small non-coding RNAs. |
WOS研究方向 | Multidisciplinary Sciences |
语种 | 英语 |
WOS记录号 | WOS:000443536100007 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3475] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Ma, Jinbiao; Chen, Xuemei; Huang, Ying |
作者单位 | 1.Fudan Univ, State Key Lab Genet Engn, Collaborat Innovat Ctr Genet & Dev, Dept Biochem,Inst Plant Biol,Sch Life Sci, Shanghai 200438, Peoples R China; 2.Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA; 3.Fudan Univ, State Key Lab Genet Engn, Dept Physiol & Biophys, Collaborat Innovat Ctr Genet & Dev,Sch Life Sci, Shanghai 200438, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,Shanghai Sci Re, CAS Ctr Excellence Mol Cell Sci,State Key Lab Mol, Natl Ctr Prot Sci Shanghai,Shanghai Key Lab Mol A, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Jiayi,Gu, Jiaqi,Ruan, Wenjie,et al. Structural and biochemical insights into small RNA 3 ' end trimming by Arabidopsis SDN1[J]. NATURE COMMUNICATIONS,2018,9(1):3585. |
APA | Chen, Jiayi.,Gu, Jiaqi.,Ruan, Wenjie.,Zhang, Lu.,Ma, Jinbiao.,...&Huang, Ying.(2018).Structural and biochemical insights into small RNA 3 ' end trimming by Arabidopsis SDN1.NATURE COMMUNICATIONS,9(1),3585. |
MLA | Chen, Jiayi,et al."Structural and biochemical insights into small RNA 3 ' end trimming by Arabidopsis SDN1".NATURE COMMUNICATIONS 9.1(2018):3585. |
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