Runt-Related Transcription Factor 1 (RUNX1) Promotes TGF-beta-Induced Renal Tubular Epithelial-to-Mesenchymal Transition (EMT) and Renal Fibrosis through the PI3K Subunit p110 delta | |
Zhou, Tong1; Luo, Maocai1,2; Zhou, Siyuan1,2; Feng, Danying1,2; Xu, Chundi1; Wang, Hongyan2; Cai, Wei3 | |
刊名 | EBIOMEDICINE |
2018 | |
卷号 | 31期号:1页码:217-225 |
关键词 | Kidney Fibrosis Signaling Pathway In-vitro Rheumatoid-arthritis Cell-proliferation Breast-cancer Differentiation Gene Inflammation Activation |
ISSN号 | 2352-3964 |
DOI | 10.1016/j.ebiom.2018.04.023 |
文献子类 | Article |
英文摘要 | Renal fibrosis is widely considered a common mechanism leading to end-stage renal failure. Epithelial-to-mesenchymal transition (EMT) plays important roles in the pathogenesis of renal fibrosis. Runt-related transcription factor 1 (RUNX1) plays a vital role in hematopoiesis via Endothelial-to-Hematopoietic Transition (EHT), a process that is conceptually similar to EMT, but its role in EMT and renal fibrosis is unclear. Here, we demonstrate that RUNX1 is overexpressed in the processes of TGF-beta-induced partial EMT and renal fibrosis and that the expression level of RUNX1 is SMAD3-dependent.Knockdown of RUNX1 attenuated both TGE-beta-induced phenotypic changes and the expression levels of EMT marker genes in renal tubular epithelial cells (RTECs). In addition, overexpression of RUNXI promoted the expression of EMT marker genes in renal tubular epithelial cells. Moreover, RUNXI promoted TGF-beta-induced partial EMT by increasing transcription of the PI3K subunit p110 delta, which mediated Akt activation. Specific deletion of Runx1 in mouse RTECs attenuated renal fibrosis, which was induced by both unilateral ureteral obstruction (UUO) and folic acid (FA) treatment. These findings suggest that RUNX1 is a potential target for preventing renal fibrosis. (C) 2018 The Authors. Published by Elsevier B.V. |
WOS研究方向 | Medicine, General & Internal ; Medicine, Research & Experimental |
语种 | 英语 |
WOS记录号 | WOS:000433078300028 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3462] |
专题 | 生化所2018年发文 |
通讯作者 | Zhou, Tong |
作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Dept Pediat, Ruijin Hosp, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,Innovat Ctr Cel, State Key Lab Cell Biol,Key Lab Syst Biol,CAS Ctr, Shanghai, Peoples R China; 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Surg, Sch Med, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Zhou, Tong,Luo, Maocai,Zhou, Siyuan,et al. Runt-Related Transcription Factor 1 (RUNX1) Promotes TGF-beta-Induced Renal Tubular Epithelial-to-Mesenchymal Transition (EMT) and Renal Fibrosis through the PI3K Subunit p110 delta[J]. EBIOMEDICINE,2018,31(1):217-225. |
APA | Zhou, Tong.,Luo, Maocai.,Zhou, Siyuan.,Feng, Danying.,Xu, Chundi.,...&Cai, Wei.(2018).Runt-Related Transcription Factor 1 (RUNX1) Promotes TGF-beta-Induced Renal Tubular Epithelial-to-Mesenchymal Transition (EMT) and Renal Fibrosis through the PI3K Subunit p110 delta.EBIOMEDICINE,31(1),217-225. |
MLA | Zhou, Tong,et al."Runt-Related Transcription Factor 1 (RUNX1) Promotes TGF-beta-Induced Renal Tubular Epithelial-to-Mesenchymal Transition (EMT) and Renal Fibrosis through the PI3K Subunit p110 delta".EBIOMEDICINE 31.1(2018):217-225. |
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