GOLPH2-regulated oncolytic adenovirus, GD55, exerts strong killing effect on human prostate cancer stem-like cells in vitro and in vivo | |
Ying, Chang1; Xiao, Bo-duan1; Qin, Yun1; Wang, Bin-rong1; Liu, Xin-yuan1,2; Zhou, Xiu-mei1; Wang, Yi-gang1; Wang, Ru-wei3; Fang, Ling3; Yan, Hui4 | |
刊名 | ACTA PHARMACOLOGICA SINICA |
2018 | |
卷号 | 39期号:3页码:405-414 |
关键词 | Potent Antitumor-activity Colorectal-cancer Gene Identification Heterogeneity Plasticity Resistance Xenograft Promoter Efficacy |
ISSN号 | 1671-4083 |
DOI | 10.1038/aps.2017.91 |
文献子类 | Article |
英文摘要 | GOLPH2 (also called GP73) is a Golgi glycoprotein, which has been identified as a novel tumor marker upregulated in various cancers, including prostate cancer (PCa). GD55 is a novel GOLPH2-regulated oncolytic adenovirus that exhibits a strong killing effect on hepatoma cells. Here, we investigate the antitumor effect of GD55 on prostate cancer stem cell (CSC)-like cells in vitro and in vivo. Prostate CSC-like sphere cells were acquired and enriched by culturing DU145, LNCap or P3 prostate cancer cells in suspension. The prostate CSC-like sphere cells were capable of self-renewal, differentiation and quiescence, displaying tumorigenic feature and chemo-resistance to 5-FU, doxorubicin and DDP. Treatment with GD55 (1, 5, 10 MOI) dose-dependently suppressed the viability of DU145 sphere cells, which was a more pronounced compared to its cytotoxic action on the parental DU145 cells. In a mouse xenograft prostate CSC-like model, intratumoral injection of GD55 markedly suppressed the growth rate of xenograft tumors and induced higher levels of cell death and necrosis within the tumor tissues. Our results demonstrate that GD55 infection exerts strong anticancer effects on prostate CSC-like cells in vitro and in vivo, and has a potential to be used in the clinical therapy of PCa. |
电子版国际标准刊号 | 1745-7254 |
WOS研究方向 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
语种 | 英语 |
WOS记录号 | WOS:000426844400008 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3413] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Wang, Yi-gang |
作者单位 | 1.Zhejiang Sci Tech Univ, Coll Life Sci, Xinyuan Inst Med & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 3.Zhejiang Conba Pharmaceut Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China; 4.Zhejiang Acad Med Sci, Hangzhou 310013, Zhejiang, Peoples R China |
推荐引用方式 GB/T 7714 | Ying, Chang,Xiao, Bo-duan,Qin, Yun,et al. GOLPH2-regulated oncolytic adenovirus, GD55, exerts strong killing effect on human prostate cancer stem-like cells in vitro and in vivo[J]. ACTA PHARMACOLOGICA SINICA,2018,39(3):405-414. |
APA | Ying, Chang.,Xiao, Bo-duan.,Qin, Yun.,Wang, Bin-rong.,Liu, Xin-yuan.,...&Yan, Hui.(2018).GOLPH2-regulated oncolytic adenovirus, GD55, exerts strong killing effect on human prostate cancer stem-like cells in vitro and in vivo.ACTA PHARMACOLOGICA SINICA,39(3),405-414. |
MLA | Ying, Chang,et al."GOLPH2-regulated oncolytic adenovirus, GD55, exerts strong killing effect on human prostate cancer stem-like cells in vitro and in vivo".ACTA PHARMACOLOGICA SINICA 39.3(2018):405-414. |
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