A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities | |
Chen, Yun1; Ruan, Zhi-Rong1; Wang, Yong1,2; Huang, Qian1; Xue, Mei-Qin1; Zhou, Xiao-Long1; Wang, En-Duo1,2 | |
刊名 | NUCLEIC ACIDS RESEARCH |
2018 | |
卷号 | 46期号:7页码:3643-3656 |
关键词 | Translational Quality-control Active-site Amino-acids Domain Trna(Leu) Nuclear Discrimination Biosynthesis Mechanism Fidelity |
ISSN号 | 0305-1048 |
DOI | 10.1093/nar/gky211 |
文献子类 | Article |
英文摘要 | TARS and TARS2 encode cytoplasmic and mitochondrial threonyl-tRNA synthetases (ThrRSs) in mammals, respectively. Interestingly, in higher eukaryotes, a third gene, TARSL2, encodes a ThrRS-like protein (ThrRS-L), which is highly homologous to cytoplasmic ThrRS but with a different N-terminal extension (N-extension). Whether ThrRS-L has canonical functions is unknown. In this work, we studied the organ expression pattern, cellular localization, canonical aminoacylation and editing activities of mouse ThrRS-L (mThrRS-L). Tarsl2 is ubiquitously but unevenly expressed in mouse tissues. Different from mouse cytoplasmic ThrRS (mThrRS), mThrRS-L is located in both the cytoplasm and nucleus; the nuclear distribution is mediated via a nuclear localization sequence at its C-terminus. Native mThrRS-L enriched from HEK293T cells was active in aminoacylation and editing. To investigate the in vitro catalytic properties of mThrRS-L accurately, we replaced the N-extension of mThrRS-L with that of mThrRS. The chimeric protein (mThrRS-L-NT) has amino acid activation, aminoacylation and editing activities. We compared the activities and cross-species tRNA recognition between mThrRS-L-NT and mThrRS. Despite having a similar aminoacylation activity, mThrRS-L-NT and mThrRS exhibit differences in tRNA recognition and editing capacity. Our results provided the first analysis of the aminoacylation and editing activities of ThrRS-L, and improved our understanding of Tarsl2. |
电子版国际标准刊号 | 1362-4962 |
WOS研究方向 | Biochemistry & Molecular Biology |
语种 | 英语 |
WOS记录号 | WOS:000431137900034 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3374] |
专题 | 生化所2018年发文 |
通讯作者 | Wang, En-Duo |
作者单位 | 1.Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol,Chinese Acad Sci, 320 Yue Yang Rd, Shanghai, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Yun,Ruan, Zhi-Rong,Wang, Yong,et al. A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities[J]. NUCLEIC ACIDS RESEARCH,2018,46(7):3643-3656. |
APA | Chen, Yun.,Ruan, Zhi-Rong.,Wang, Yong.,Huang, Qian.,Xue, Mei-Qin.,...&Wang, En-Duo.(2018).A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities.NUCLEIC ACIDS RESEARCH,46(7),3643-3656. |
MLA | Chen, Yun,et al."A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities".NUCLEIC ACIDS RESEARCH 46.7(2018):3643-3656. |
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