A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction | |
Fan, Qingqian1; Guan, Yueping1; Zhang, Zhi1; Xu, Guoli1; Yang, Yu3; Guo, Chen2 | |
刊名 | CHEMICAL PHYSICS LETTERS
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2019 | |
卷号 | 715页码:7-13 |
关键词 | Magnetic nanoparticles Core-shell DNA extraction Fe3O4@SiO2 |
ISSN号 | 0009-2614 |
DOI | 10.1016/j.cplett.2018.11.001 |
英文摘要 | A new method was developed for preparing monodisperse Fe3O4@SiO2 magnetic core-shell nanoparticles via three steps. The obtained nanoparticles exhibit high saturation magnetization, good surface topography and dispersion as characterized by FTIR, SEM and VSM. The experimental results of hydrochloric acid treatment show that the obtained nanoparticles have excellent acid resistance and stable and compact silicon coating. The magnetic core-shell nanoparticles were further tested for purification of plasmid DNA from E. coli DH5a cells. The result of purification leaded to better adsorption, elution and purity results in comparison with isolated commercial extraction kit. |
资助项目 | National Key Research and Development Program of China[2018YFF0214900] |
WOS关键词 | GENOMIC DNA ; SILICA |
WOS研究方向 | Chemistry ; Physics |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000455097200002 |
资助机构 | National Key Research and Development Program of China |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/27713] ![]() |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Guan, Yueping |
作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China |
推荐引用方式 GB/T 7714 | Fan, Qingqian,Guan, Yueping,Zhang, Zhi,et al. A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction[J]. CHEMICAL PHYSICS LETTERS,2019,715:7-13. |
APA | Fan, Qingqian,Guan, Yueping,Zhang, Zhi,Xu, Guoli,Yang, Yu,&Guo, Chen.(2019).A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction.CHEMICAL PHYSICS LETTERS,715,7-13. |
MLA | Fan, Qingqian,et al."A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction".CHEMICAL PHYSICS LETTERS 715(2019):7-13. |
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