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A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction
Fan, Qingqian1; Guan, Yueping1; Zhang, Zhi1; Xu, Guoli1; Yang, Yu3; Guo, Chen2
刊名CHEMICAL PHYSICS LETTERS
2019
卷号715页码:7-13
关键词Magnetic nanoparticles Core-shell DNA extraction Fe3O4@SiO2
ISSN号0009-2614
DOI10.1016/j.cplett.2018.11.001
英文摘要A new method was developed for preparing monodisperse Fe3O4@SiO2 magnetic core-shell nanoparticles via three steps. The obtained nanoparticles exhibit high saturation magnetization, good surface topography and dispersion as characterized by FTIR, SEM and VSM. The experimental results of hydrochloric acid treatment show that the obtained nanoparticles have excellent acid resistance and stable and compact silicon coating. The magnetic core-shell nanoparticles were further tested for purification of plasmid DNA from E. coli DH5a cells. The result of purification leaded to better adsorption, elution and purity results in comparison with isolated commercial extraction kit.
资助项目National Key Research and Development Program of China[2018YFF0214900]
WOS关键词GENOMIC DNA ; SILICA
WOS研究方向Chemistry ; Physics
语种英语
出版者ELSEVIER SCIENCE BV
WOS记录号WOS:000455097200002
资助机构National Key Research and Development Program of China
内容类型期刊论文
源URL[http://ir.ipe.ac.cn/handle/122111/27713]  
专题中国科学院过程工程研究所
通讯作者Guan, Yueping
作者单位1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China
推荐引用方式
GB/T 7714
Fan, Qingqian,Guan, Yueping,Zhang, Zhi,et al. A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction[J]. CHEMICAL PHYSICS LETTERS,2019,715:7-13.
APA Fan, Qingqian,Guan, Yueping,Zhang, Zhi,Xu, Guoli,Yang, Yu,&Guo, Chen.(2019).A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction.CHEMICAL PHYSICS LETTERS,715,7-13.
MLA Fan, Qingqian,et al."A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction".CHEMICAL PHYSICS LETTERS 715(2019):7-13.
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