Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli

	Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
	Lee, Taek Soon; Liu, Chun-Li; Tian, Tian; Alonso-Gutierrez, Jorge; Garabedian, Brett; Wang, Shuai; Baidoo, Edward E. K.; Benites, Veronica; Chen, Yan; Petzold, Christopher J.
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2018
文献子类	期刊论文
英文摘要	BackgroundAviation fuels are an important target of biofuels research due to their high market demand and competitive price. Isoprenoids have been demonstrated as good feedstocks for advanced renewable jet fuels with high energy density, high heat of combustion, and excellent cold-weather performance. In particular, sesquiterpene compounds (C-15), such as farnesene and bisabolene, have been identified as promising jet fuel candidates.ResultsIn this study, we explored three sesquiterpenesepi-isozizaene, pentalenene and -isocomeneas novel jet fuel precursors. We performed a computational analysis to calculate the energy of combustion of these sesquiterpenes and found that their specific energies are comparable to commercial jet fuel A-1. Through heterologous MVA pathway expression and promoter engineering, we produced 727.9mg/L epi-isozizaene, 780.3mg/L pentalenene and 77.5mg/L -isocomene in Escherichia coli and 344mg/L pentalenene in Saccharomyces cerevisiae. We also introduced a dynamic autoinduction system using previously identified FPP-responsive promoters for inducer-free production and managed to achieve comparable amounts of each compound.ConclusionWe produced tricyclic sesquiterpenes epi-isozizaene, pentalenene and -isocomene, promising jet fuel feedstocks at high production titers, providing novel, sustainable alternatives to petroleum-based jet fuels.
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/13436]
专题	合成所
推荐引用方式 GB/T 7714	Lee, Taek Soon,Liu, Chun-Li,Tian, Tian,et al. Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli[J]. BIOTECHNOLOGY FOR BIOFUELS,2018.
APA	Lee, Taek Soon.,Liu, Chun-Li.,Tian, Tian.,Alonso-Gutierrez, Jorge.,Garabedian, Brett.,...&Tan, Tianwei.(2018).Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli.BIOTECHNOLOGY FOR BIOFUELS.
MLA	Lee, Taek Soon,et al."Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli".BIOTECHNOLOGY FOR BIOFUELS (2018).