Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae.

	Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae.
	Hou, Sha; Dai, Junbiao; Qin, Qin
刊名	ACS SYNTHETIC BIOLOGY
	2018
文献子类	期刊论文
英文摘要	Yeast can be used as a microbial cell factory to produce valuable chemicals. However, introducing an exogenous pathway into particular or different chromosomal locations for stable expression is still a daunting task. To address this issue, we designed a DNA cassette called a "wicket", which can be integrated into the yeast genome at designated loci to accept exogenous DNA upon excision by a nuclease. Using this system, we demonstrated that, instrains with "wickets", we could achieve near 100% efficiency for integration of the beta-carotene pathway with no need for selective markers. Furthermore, it allowed independent and simultaneous integration of different genes in a pathway, resulting in a large variety of strains with variable copy numbers ofeach gene. This system could be a useful tool to modulate the integration of multiple copies of genes within a metabolic pathway and to optimize the yield ofthe target products.
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/13422]
专题	合成所
推荐引用方式 GB/T 7714	Hou, Sha,Dai, Junbiao,Qin, Qin. Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae.[J]. ACS SYNTHETIC BIOLOGY,2018.
APA	Hou, Sha,Dai, Junbiao,&Qin, Qin.(2018).Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae..ACS SYNTHETIC BIOLOGY.
MLA	Hou, Sha,et al."Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae.".ACS SYNTHETIC BIOLOGY (2018).