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	Tracking nonenveloped viruses modified by amino acid metabolism in cells through in situ bioorthogonal labeling strategy
	Lintao Cai; Fangfang Wang; Yutong Han; Hong Pan; Yingmei Luo; Yifan Man
	2018
会议日期	2018
会议地点	上海
英文摘要	Enterovirus 71 (EV71) is a major agent of hand, foot and mouth disease (HFMD), which can progress to severe neurological disease and death. Tracking virus infection routes in live cells is highly important for understanding the dynamic and mechanism of virus infection. However, it’s rather difficult to track EV71 in cells without weakening the viral infectivity using traditional strategies, owing to the nonenveloped structure and less than 40nmsize. Herein, we develop the in situ bioorthogonal labeling strategy based on the amino acid metabolismto track nonenveloped viruses in cells.First, EV71 capsid is modified with L-Azidohomoalanine (N3-EV71)through metabolic engineering. Then, N3-EV71 particles are in situ bioorthogonal labeled with dibenzocyclooctyl (DBCO)-functionalized fluorescenceto track the infection route.The results show that the neither the azido modification on EV71 dampen the viral infectivity, nor the DBCO-probe in-situ labelled on N3-EV71 particles weaken the viral infectivity.And the living imaging shows N3-EV71 particles can be conjugated by DBCO-probe within 30min.Furthermore, the EV71 viral infection eventsare presented systematically with a precise time. Significantly, the process of viral RNA released from the capsid is captured accuratelyin living imaging. Hence, the in-situ bioorthogonal labeling has a promising future in tracking nonenveloped viruses in cells
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14746]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Lintao Cai,Fangfang Wang,Yutong Han,et al. Tracking nonenveloped viruses modified by amino acid metabolism in cells through in situ bioorthogonal labeling strategy[C]. 见:. 上海. 2018.

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