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	Paired bioorthogonalglycometabolic labeling for enhancing T cell targeting and cytotoxicity against lymphoma cells
	Wenjun Li; Zhihong Sun; Guanjun Deng; Ping Gong; Hong Pan; Xiaoqing Meng; Zhenguo Liang; Xin Jiang; Lintao Cai; Qian Ren
	2018
会议日期	2018
会议地点	上海
英文摘要	To effectively enhance affinity of T cells to lymphoma cells and improve its cytotoxicity, we describe a paired bioorthogonal strategy for labeling of glycans in tumor cells and active T cells through metabolic process of unnatural sugars containing bicycle [6.1.0] nonyne (BCN) or azide motifs, respectively. The new bifunctional unnatural sugars, especially BCN-Man, have been developed for efficient and nondestructive incorporation of the chemical reporters into wide cellular surface glycans, where the BCN motif exhibited an excellent bioorthogonal targeting and rapid reaction rate. Next, the complementary functional groups (-N3 / -BCN) were successfully incorporated into active T cells and B lymphoma cells through glycometabolic engineering of Ac4GalAz and BCN-Man, respectively. Subsequently, the results of cell binding assay showed that complementary functional group modification remarkably enhanced the targeting and migration of T cells for lymphoma cells. Simultaneously, we also found that the cytotoxicity of azido T cell against BCN-lymphoma cell increased by 2.7 times, when compared to the unlabeled control cells. These results demonstrated the promising potential of based complementary bioorthogonalglycometabolic labeling for various applications ranging from T cell immunotherapy in clinical to studying cell-cell interactions in vivo
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14745]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Wenjun Li,Zhihong Sun,Guanjun Deng,et al. Paired bioorthogonalglycometabolic labeling for enhancing T cell targeting and cytotoxicity against lymphoma cells[C]. 见:. 上海. 2018.

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