VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.

	VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.
	Zhong-Lin Xiao; Songjun Li; Yan Yu; Mengxia Li; Jie Chen; Feng Wang; Jian Zhang; Weifen Deng; Qing Yang; Xiujun Fan
刊名	Molecular and Cellular Biochemistry
	2018
文献子类	期刊论文
英文摘要	Studies have shown that sFlt-1 overproduction stimulated by excess VEGF of deciduous origin in trophoblasts can cause preeclampsia. However, the mechanism underlying how VEGF regulates sFtl-1 expression in trophoblasts remains unknown. To address this issue, JEG3 and HTR-8/SV neo (HTR8) trophoblast cell lines were used to investigate the signaling pathways involved in the regulation of sFlt-1 production via VEGF overexpression in vitro. JEG3 (VEGF–GFP–JEG3, V-J) and HTR8 (VEGF–GFP–HTR8, V-H) cells overexpressing VEGF165 were established by infecting the JEG3 and HTR8 cell lines with lentivirus expressing VEGF165. Both the mRNA and protein levels of VEGF and sFlt-1 were dramatically up-regulated in the V-J and V-H cells compared to the JEG3 and HTR8 cells, and they were significantly decreased after treatment with an Flt-1 receptor inhibitor (MK-2461), a KDR receptor inhibitor (XL-184), or an Flt-1 and KDR receptor inhibitor (ABT-869). The mRNA levels of sFlt-1, Flt-1, and KDR were increased in V-H cells after treatment, and the VEGF-A mRNA levels were also elevated. The migration and invasion abilities of JEG3 and HTR8 cells were decreased after VEGF overexpression, and this reduction could be reversed with VEGF receptor inhibitor treatment. In addition, after the different treatments, the cell migration rates of V-J cells were significantly increased compared with the control treatment. Taken together, these results indicate that sFlt-1 up-regulation by VEGF may be mediated by the VEGF/Flt-1 and/or VEGF/KDR signaling pathways. However, elucidating which pathway plays this key role requires further investigatio
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14690]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Zhong-Lin Xiao,Songjun Li,Yan Yu,et al. VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.[J]. Molecular and Cellular Biochemistry,2018.
APA	Zhong-Lin Xiao.,Songjun Li.,Yan Yu.,Mengxia Li.,Jie Chen.,...&Xiujun Fan.(2018).VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors..Molecular and Cellular Biochemistry.
MLA	Zhong-Lin Xiao,et al."VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.".Molecular and Cellular Biochemistry (2018).