A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

	A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
	Xue-Feng Yu; Paul K. Chu; Zhen Zhao; Liming Ying; Li Hu; Wenhua Zhou
刊名	Nature COMMUNICATIONS
	2018
文献子类	期刊论文
英文摘要	Although polymerase chain reaction (PCR) is the most widely used method for DNA amplification, the requirement of thermocycling limits its non-laboratory applications. Isothermal DNA amplification techniques are hence valuable for on-site diagnostic applications in place of traditional PCR. Here we describe a true isothermal approach for amplifying and detecting double-stranded DNA based on a CRISPR–Cas9-triggered nicking endonuclease-mediated Strand Displacement Amplification method (namely CRISDA). CRISDA takes advantage of the high sensitivity/specificity and unique conformational rearrangements of CRISPR effectors in recognizing the target DNA. In combination with a peptide nucleic acid (PNA) invasion-mediated endpoint measurement, the method exhibits attomolar sensitivity and single-nucleotide specificity in detection of various DNA targets under a complex sample background. Additionally, by integrating the technique with a Cas9-mediated target enrichment approach, CRISDA exhibits sub-attomolar sensitivity. In summary, CRISDA is a powerful isothermal tool for ultrasensitive and specific detection of nucleic acids in point-of-care diagnostics and field analyses.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14669]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Xue-Feng Yu,Paul K. Chu,Zhen Zhao,et al. A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection[J]. Nature COMMUNICATIONS,2018.
APA	Xue-Feng Yu,Paul K. Chu,Zhen Zhao,Liming Ying,Li Hu,&Wenhua Zhou.(2018).A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection.Nature COMMUNICATIONS.
MLA	Xue-Feng Yu,et al."A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection".Nature COMMUNICATIONS (2018).