QQ客服 官方微博 反馈留言

	Optofluidic devices for single-step miRNA detection using molecular probes
	Hongtao Feng; Zongwen Jin; Yan Chen
	2018
会议日期	2018
会议地点	上海
英文摘要	MicroRNAs(miRNAs) have attracted extensive interest as novel biomarkers in diagnosis and targeted treatment of many diseases, and quantification of miRNAs can be potentially useful for early disease detection, surveillance monitoring and drug response evaluation. Because of the short length, high sequence similarity and low abundance of miRNAs in vivo, it is challenging to realize the sensitive and selective detection of miRNAs with conventional methods. Here, we developed an innovative fiber-based optofluidic platform to perform miRNA identification and quantification using molecular probes. The optofluidic device integrated optical fibers and on-chip microlens, and the compact design increased the detection sensitivity and eliminated time consuming optical alignments. First, we demonstrated the on-chip miRNA detection using fluorescence resonance energy transfer (FRET) principle. A three-way junction assembled FRET probes were encapsulated in droplets to carry out ratiometric miRNA detection with highly selective discrimination of single-base mismatch. To improve the sensitivity of the detection system, we introduced another molecular probe in the optofluidic system using bioluminescence resonance energy transfer (BRET) principle, in which the donor fluorophore is replaced with a self-illuminating luciferase. To carry out the miRNA detection steps, the optofluidic device integrated a variety of components, such as droplet pico-injection unit and high performance fiber-based optical detection unit, and single-step miRNA detection can be performed with simple device operation. In summary, the compact optofluidic system provides a low-cost and highly efficient approach for miRNA identification and quantification with high sensitivity and specificity, and has great potential in point-of-care clinical diagnostics.
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14530]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Hongtao Feng,Zongwen Jin,Yan Chen. Optofluidic devices for single-step miRNA detection using molecular probes[C]. 见:. 上海. 2018.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。