A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing

	A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing
	Randy P. Carney; Tingrui Pan; Kit S. Lam; Yuanpei Li; Yongfan Men; Yousif Ajena; Gerald Quon; Jiannan Li; Wen Tan; Wenwu Xiao
刊名	Analytical Chemistry
	2018
文献子类	期刊论文
英文摘要	Traditional high-throughput drug combination screening requires automatic pipetting of drugs into high-density microtiter plates. Here, a drug-on-pillar platform is proposed for efficient combination drug screening. Using the proposed approach, combination drug screening can be carried out in a plug-and-play manner, allowing for high-throughput screening of large permutations of drug combinations at various concentrations, such that drug dispensing and cell-based screening can be temporally separated and therefore can potentially be performed at distant laboratories. The dispensing is implemented using our recently developed microfluidic pneumatic printing platform, which features a low-cost disposable cartridge that minimizes cross contamination. Moreover, our previously developed drug nanoformulation method with amphiphilic telodendrimers has been utilized to maintain drug stability in a dry form, allowing for convenient drug storage, shipping, and subsequent rehydration. Combining the features described above, we have implemented a 1260-spot drug combination array to study the effect of paired drugs against MDA-MB-231 triple negative human breast cancer cells. This study supports the feasibility of the drug-on-pillar platform for combination drug screening and has provided valuable insight into drug combination efficacy against breast cancer.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14445]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Randy P. Carney,Tingrui Pan,Kit S. Lam,et al. A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing[J]. Analytical Chemistry,2018.
APA	Randy P. Carney.,Tingrui Pan.,Kit S. Lam.,Yuanpei Li.,Yongfan Men.,...&Wenwu Xiao.(2018).A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing.Analytical Chemistry.
MLA	Randy P. Carney,et al."A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing".Analytical Chemistry (2018).