Sorting of tumour cells in a microfluidic device by multi-stage surface acoustic waves.

	Sorting of tumour cells in a microfluidic device by multi-stage surface acoustic waves.
	Meng, Long; Niu, Lili; Zheng, Hairong; Wang, Kaiyue; Zhou, Wei; Lin, Zhengrong; Cai, Feiyan; Li, Fei; Wu, Junru
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018
文献子类	期刊论文
英文摘要	Detection of circulating tumour cells (CTCs) plays a crucial role in the diagnosis of tumour metastasis and evaluation of therapeutic effect on prognosis. The low number of the CTCs, however, makes their isolation from the peripheral blood very difficult. In this paper, a microfluidic device has been developed, which consists of a pair of straight interdigital transducers (IDTs) and focused interdigital transducers (FIDTs), to separate tumour cells from red blood cells(RBCs) in the blood stream by surface acoustic waves (SAWs). The standing SAWs (SSAW) generated by the straight IDTs enabled placing the CTCs and RBCs at the pressure nodes while the FIDTs generated the focused travelling pulsed SAWs (TSAWs) to push the CTCs away from the blood cells to achieve the isolation of CTCs. Using this mechanism, this device was capable of concentrating and separating 2 mu m and 5 mu m polystyrene particles and about 94.2% ofparticles with the diameter of 5 mu m could be collected at the outlet. Moreover, 90% +/- 2.4% of U87 glioma cells could be isolated from the RBCs when the cycle number of the TSAWs was 720 k. In the enrichment process, this microfluidic device totally relied on the SSAW without any additional assistance of the flow focusing. For the separation, unlike the bidirectional acoustic radiation force in SSAW, the unidirectional force generated by TSAWs consistently pushed the particles along the wave propagation, improving the sorting performance. This multi-stage device may provide an alternative method for sorting CTCs ina reliable and flexible manner.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14317]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Meng, Long,Niu, Lili,Zheng, Hairong,et al. Sorting of tumour cells in a microfluidic device by multi-stage surface acoustic waves.[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018.
APA	Meng, Long.,Niu, Lili.,Zheng, Hairong.,Wang, Kaiyue.,Zhou, Wei.,...&Wu, Junru.(2018).Sorting of tumour cells in a microfluidic device by multi-stage surface acoustic waves..SENSORS AND ACTUATORS B-CHEMICAL.
MLA	Meng, Long,et al."Sorting of tumour cells in a microfluidic device by multi-stage surface acoustic waves.".SENSORS AND ACTUATORS B-CHEMICAL (2018).