Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds.

	Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds.
	Liu, Chaoyu; Wang, Chong; Zhao, Qilong; Li, Xiaohua; Xu, Feiyue; Yao, Xumei; Wang, Min
刊名	BIOMEDICAL MATERIALS
	2018
文献子类	期刊论文
英文摘要	Electrospun fibrous scaffolds have been extensively used as cell-supporting matrices or delivery vehicles for various biomolecules in tissue engineering. Biodegradable scaffolds with tunable degradation behaviors are favorable for various resorbable tissue replacements. In nerve tissue engineering, delivery of growth factors (GFs) such as nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) from scaffolds can be used to promote peripheral nerve repair. In this study, using the established dual-source dual-power electrospinning technique, bicomponent scaffolds incorporated with NGF and GDNF were designed and demonstrated as a strategy to develop scaffolds providing dual GF delivery. NGF and GDNF were encapsulated in poly(D, L-lactic acid) (PDLLA) and poly(lactic-co-glycolic acid) (PLGA) nanofibers, respectively, via emulsion Kelectrospinning. Bicomponent scaffolds with various mass ratios of GDNF/PLGA fibers to NGF/PDLLA fibers were fabricated. Their morphology, structure, properties, and the in vitro degradation were examined. Both types of core-shell structured fibers were evenly distributed in bicomponent scaffolds. Robust scaffolds with varying component ratios were fabricated with average fiber diameter ranging from 307 +/- 100 nm to 688 +/- 129 nm. The ultimate tensile stress and elastic modulus could be tuned ranging from 0.23 +/- 0.07 MPa to 1.41 +/- 0.23 MPa, 11.1 +/- 3.0 MPa to 75.9 +/- 3.3 MPa, respectively. Adjustable degradation was achieved and the weight loss of scaffoldsranged from 9.2% to 44.0% after 42 day degradation test. GDNF and NGF were incorporated with satisfactory encapsulation efficiency and their bioactivity were well preserved. Sustained release of both types of GFs was also achieved.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14269]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Liu, Chaoyu,Wang, Chong,Zhao, Qilong,et al. Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds.[J]. BIOMEDICAL MATERIALS,2018.
APA	Liu, Chaoyu.,Wang, Chong.,Zhao, Qilong.,Li, Xiaohua.,Xu, Feiyue.,...&Wang, Min.(2018).Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds..BIOMEDICAL MATERIALS.
MLA	Liu, Chaoyu,et al."Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds.".BIOMEDICAL MATERIALS (2018).