Flexibly adjustable depth-of-focus photoacoustic microscopy with spatial light modulation

	Flexibly adjustable depth-of-focus photoacoustic microscopy with spatial light modulation
	Zheng, Wei; Song, Liang; Yuan, Xiaocong; Song, Wei; Wu, Yuli; Gao, Yufeng; Chen, Tingai; Fang, Hui
刊名	APPLIED PHYSICS LETTERS
	2018
文献子类	期刊论文
英文摘要	Through focusing the excitation laser, optical-resolution photoacoustic microscopy (OR-PAM) is capable of measuring optical absorption properties down to micrometer-scale lateral resolution within biological tissues. The focused Gaussian beam routinely employed in the OR-PAM setups is inadequate for acquiring the volumetric images of biological specimens with thickness from tens micrometers to millimeter without scanning in depth because of the inconsistent lateral resolution along the depth direction due to its short depth of focus (DoF). Here, we integrate a spatial light modulator (SLM) into the optical path of an OR-PAM for realizing the flexibly adjustable DoF. By simply switching the phase patterns assigned onto the SLM interface, three representative illumination beams are produced, including conventional short-DoF Gaussian beam (GB), needle-like Bessel beam (BB), and extended depth-of-focus beam (EDFB). These modulations can be well realized based on the extended Nijboer-Zernike theory. The photoacoustic excitations show variable DoFs ranging from hundreds of micrometers (GB and BB) up to 1.38 mm (EDFB) but a consistent lateral resolution of similar to 3.5 mu m. The proposed method is confirmed by volumetric imaging of multiple tungsten fibers positioned at different depths. Published by AIP Publishing.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14222]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Zheng, Wei,Song, Liang,Yuan, Xiaocong,et al. Flexibly adjustable depth-of-focus photoacoustic microscopy with spatial light modulation[J]. APPLIED PHYSICS LETTERS,2018.
APA	Zheng, Wei.,Song, Liang.,Yuan, Xiaocong.,Song, Wei.,Wu, Yuli.,...&Fang, Hui.(2018).Flexibly adjustable depth-of-focus photoacoustic microscopy with spatial light modulation.APPLIED PHYSICS LETTERS.
MLA	Zheng, Wei,et al."Flexibly adjustable depth-of-focus photoacoustic microscopy with spatial light modulation".APPLIED PHYSICS LETTERS (2018).