Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-beta/Smad Pathway and Osseointegration in Osteopenic Rabbits

doi:10.1038/s41598-017-03661-5

	Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-beta/Smad Pathway and Osseointegration in Osteopenic Rabbits
	Yu, Jiangming 4; Xu, Lizhang ; Li, Kai 1; Xie, Ning ; Xi, Yanhai ; Wang, Yang ; Zheng, Xuebin 1; Chen, Xiongsheng ; Wang, Meiyan 2,3; Ye, Xiaojian
刊名	SCIENTIFIC REPORTS
	2017
卷号	7
ISSN号	2045-2322
DOI	10.1038/s41598-017-03661-5
英文摘要	Surface-modified metal implants incorporating different ions have been employed in the biomedical field as bioactive dental implants with good osseointegration properties. However, the molecular mechanism through which surface coatings exert the biological activity is not fully understood, and the effects have been difficult to achieve, especially in the osteopenic bone. In this study, We examined the effect of zinc-modified calcium silicate coatings with two different Zn contents to induce osteogenic differentiation of rat bone marrow-derived pericytes (BM-PCs) and osteogenetic efficiency in ovariectomised rabbits. Ti-6Al-4V with zinc-modified calcium silicate coatings not only enhanced proliferation but also promoted osteogenic differentiation and mineralized matrix deposition of rat BM-PCs as the zinc content and culture time increased in vitro. The associated molecular mechanisms were investigated by Q-PCR and Western blotting, revealing that TGF-beta/Smad signaling pathway plays a direct and significant role in regulating BM-PCs osteoblastic differentiation on Zn-modified coatings. Furthermore, in vivo results that revealed Zn-modified calcium silicate coatings significantly promoted new bone formation around the implant surface in osteopenic rabbits as the Zn content and exposure time increased. Therefore, Zn-modified calcium silicate coatings can improve implant osseointegration in the condition of osteopenia, which may be beneficial for patients suffering from osteoporosis-related fractures.
WOS记录号	WOS:000403140000111
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/26669]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Second Mil Med Univ, Dept Orthopaed, Changzheng Hosp, Shanghai 200003, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, Key Lab Inorgan Coating Mat, Shanghai 200050, Peoples R China 3.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai 200241, Peoples R China 4.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China 5.Natl Engn Res Ctr Nanotechnol, Shanghai 200241, Peoples R China
推荐引用方式 GB/T 7714	Yu, Jiangming,Xu, Lizhang,Li, Kai,et al. Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-beta/Smad Pathway and Osseointegration in Osteopenic Rabbits[J]. SCIENTIFIC REPORTS,2017,7.
APA	Yu, Jiangming.,Xu, Lizhang.,Li, Kai.,Xie, Ning.,Xi, Yanhai.,...&Ye, Xiaojian.(2017).Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-beta/Smad Pathway and Osseointegration in Osteopenic Rabbits.SCIENTIFIC REPORTS,7.
MLA	Yu, Jiangming,et al."Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-beta/Smad Pathway and Osseointegration in Osteopenic Rabbits".SCIENTIFIC REPORTS 7(2017).