Phenanthrene-Induced Apoptosis and Its Underlying Mechanism

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Phenanthrene-Induced Apoptosis and Its Underlying Mechanism
	Zha, Jinmiao; Chen, Rui; Qin, Jianhui; Hong, Xiangsheng; Wang, Zijian; Ji, Xiaoya; Li, Na; Huang, Chao; Yuan, Lilai
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2017-12-19
卷号	51 期号:24 页码:14397-14405
英文摘要	Phenanthrene (Phe) is one of the most abundant low-molecular-weight polycyclic aromatic hydrocarbons, (PAHs). Widespread human and aquatic organism exposure to Phe has been reported, but the toxic effects of Phe and potential mechanisms are unclear. We focused on the chronic hepatotoxicity of Phe in adult Chinese rare minnows (Gobiocypris rants) and the underlying mechanisms. The chronic effects of exposing Chinese rare minnows to 8.9, 82.3, or 510.0 mu g/L Phe for 30 days were examined by histopathological observation, TUNEL assays, caspase activity assays, and gene expression profiles. The liver lesion frequency and hepatocyte apoptosis were increased in Phe-exposed groups. Caspase 9 and caspase 3 enzyme activity in liver tissues was markedly increased. The expression of miR-17/92 cluster members was significantly increased in the 82.3 and 510.0 mu g/L groups. Moreover, the response of primary hepatocytes indicated a significant decrease in the mitochondria' membrane potential (MMP) after a 48 h exposure to Phe. Interestingly, miR-18a was significantly decreased in primary hepatocytes in all treatments. Moreover, molecular docking indicated that Phe might have the same binding domain as pri-miR-18a, forming pi-pi and pi-sigma interactions with heterogeneous nuclear ribonucleoprotein (hnRNP) Al. Given the above, Phe caused liver lesions and induced hepatocyte apoptosis through the intrinsic apoptosis pathway, and the interaction of Phe with hnRNP Al contributes to the suppression of miR-18a expression and hepatocyte apoptosis.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39727]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Zha, Jinmiao,Chen, Rui,Qin, Jianhui,et al. Phenanthrene-Induced Apoptosis and Its Underlying Mechanism[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2017,51(24):14397-14405.
APA	Zha, Jinmiao.,Chen, Rui.,Qin, Jianhui.,Hong, Xiangsheng.,Wang, Zijian.,...&Yuan, Lilai.(2017).Phenanthrene-Induced Apoptosis and Its Underlying Mechanism.ENVIRONMENTAL SCIENCE & TECHNOLOGY,51(24),14397-14405.
MLA	Zha, Jinmiao,et al."Phenanthrene-Induced Apoptosis and Its Underlying Mechanism".ENVIRONMENTAL SCIENCE & TECHNOLOGY 51.24(2017):14397-14405.