Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China
	An, Xin-Li; Gillings, Michael R.; Chen, Hong; Zhang, Tong; Zhu, Yong-Guan; Chen, Qing-Lin; Li, Bing; Su, Jian-Qiang
刊名	MICROBIOME
	2017-07-19
卷号	5 期号:0 页码:-
关键词	Antibiotic Resistance Environment Pollution Evolution Human Gut Microbiome
英文摘要	Background: Antibiotic-resistant pathogens are challenging treatment of infections worldwide. Urban sewage is potentially a major conduit for dissemination of antibiotic resistance genes into various environmental compartments. However, the diversity and abundance of such genes in wastewater are not well known. Methods: Here, seasonal and geographical distributions of antibiotic resistance genes and their host bacterial communities from Chinese urban sewage were characterized, using metagenomic analyses and 16S rRNA gene-based Illumina sequencing, respectively. Results: In total, 381 different resistance genes were detected, and these genes were extensively shared across China, with no geographical clustering. Seasonal variation in abundance of resistance genes was observed, with average concentrations of 3.27 x 10(11) and 1.79 x 10(12) copies/L in summer and winter, respectively. Bacterial communities did not exhibit geographical clusters, but did show a significant distance-decay relationship (P < 0.01). The core, shared resistome accounted for 57.7% of the total resistance genes, and was significantly associated with the core microbial community (P < 0.01). The core human gut microbiota was also strongly associated with the shared resistome, demonstrating the potential contribution of human gut microbiota to the dissemination of resistance elements via sewage disposal. Conclusions: This study provides a baseline for investigating environmental dissemination of resistance elements and raises the possibility of using the abundance of resistance genes in sewage as a tool for antibiotic stewardship.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39597]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	An, Xin-Li,Gillings, Michael R.,Chen, Hong,et al. Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China[J]. MICROBIOME,2017,5(0):-.
APA	An, Xin-Li.,Gillings, Michael R..,Chen, Hong.,Zhang, Tong.,Zhu, Yong-Guan.,...&Su, Jian-Qiang.(2017).Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China.MICROBIOME,5(0),-.
MLA	An, Xin-Li,et al."Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China".MICROBIOME 5.0(2017):-.