In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells

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	In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells
	Cai, Xiaolin; Cui, Yanshan; Sun, Guoxin; Chen, Xiaochen; Li, Zejiao; Zhang, Zhennan; Du, Huili; Yin, Naiyi
刊名	CHEMOSPHERE
	2017-02-01
卷号	168 期号:0 页码:358-364
关键词	Arsenic Speciation Gut Microbiota Caco-2 Cell Soil
英文摘要	Arsenic (As) speciation is essential in assessing health risks from As-contaminated soil. Release of soil bound arsenic, As transformation by human gut microbiota, and the subsequent intestinal absorption of soil As metabolites were evaluated. A colon microbial community in a dynamic human gut model and the intestinal epithelial cell line Caco-2 were cultured. Arsenic speciation analysis and absorption of different As species were undertaken. In this study, soil As release (3.7-581.2 mg kg(-1)) was observed in the colon. Arsenic in the colon digests was transformed more quickly than that in the soil solid phase. X-ray absorption near-edge spectroscopy (XANES) analysis showed that 44.2-97.6% of arsenite [As(III)] generated due to arsenate [As(V)] reduction was in the soil solid phase after the colon phase. We observed a high degree of cellular absorption of soil As metabolites, exhibiting that the intestinal absorption of monomethylarsonic acid and As(III) (33.6% and 30.2% resp.) was slightly higher than that of dimethylarsinic acid and As(V) (25.1% and 21.7% resp.). Our findings demonstrate that human gut microbiota can directly release soil-bound arsenic, particularly As-bearing amorphous Fe/Al-oxides. Determining As transformation and intestinal absorption simultaneously will result in an accurate risk assessment of human health with soil As exposures. (C) 2016 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39590]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Cai, Xiaolin,Cui, Yanshan,Sun, Guoxin,et al. In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells[J]. CHEMOSPHERE,2017,168(0):358-364.
APA	Cai, Xiaolin.,Cui, Yanshan.,Sun, Guoxin.,Chen, Xiaochen.,Li, Zejiao.,...&Yin, Naiyi.(2017).In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells.CHEMOSPHERE,168(0),358-364.
MLA	Cai, Xiaolin,et al."In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells".CHEMOSPHERE 168.0(2017):358-364.