A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus
	Zhang, Yinfeng; Li, Yuanyuan; Qin, Zhanfen; Wang, Huili; Li, Jianzhong
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2015-08-01
卷号	34 页码:143-154
关键词	Thyroid Hormone Signaling Disruptor Pelophylax Nigromaculatus Th-response Gene Expression Screening Assay Tetrabromobisphenol a
英文摘要	Amphibian metamorphosis provides a wonderful model to study the thyroid hormone (TH) signaling disrupting activity of environmental chemicals, with Xenopus laevis as the most commonly used species. This study aimed to establish a rapid and sensitive screening assay based on TH-response gene expression analysis using Pelophylax nigromaculatus, a native frog species distributed widely in East Asia, especially in China. To achieve this, five candidate TH-response genes that were sensitive to T3 induction were chosen as molecular markers, and T3 induction was determined as 0.2 nmol/L T3 exposure for 48 hr. The developed assay can detect the agonistic activity of T3 with a lowest observed effective concentration of 0.001 nmol/L and EC50 at around 0.118-1.229 nmol/L, exhibiting comparable or higher sensitivity than previously reported assays. We further validated the efficiency of the developed assay by detecting the TH signaling disrupting activity of tetrabromobisphenol A (TBBPA), a known TH signaling disruptor. In accordance with previous reports, we found a weak TH agonistic activity for TBBPA in the absence of T3, whereas a TH antagonistic activity was found for TBBPA at higher concentrations in the presence of T3, showing that the P. nigromaculatus assay is effective for detecting TH signaling disrupting activity. Importantly, we observed non-monotonic dose-dependent disrupting activity of TBBPA in the presence of T3, which is difficult to detect with in vitro reporter gene assays. Overall, the developed P. nigromaculatus assay can be used to screen TH signaling disrupting activity of environmental chemicals with high sensitivity. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
WOS研究方向	Environmental Sciences
WOS记录号	WOS:000362980400016
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32908]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Zhang, Yinfeng,Li, Yuanyuan,Qin, Zhanfen,et al. A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2015,34:143-154.
APA	Zhang, Yinfeng,Li, Yuanyuan,Qin, Zhanfen,Wang, Huili,&Li, Jianzhong.(2015).A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus.JOURNAL OF ENVIRONMENTAL SCIENCES,34,143-154.
MLA	Zhang, Yinfeng,et al."A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus".JOURNAL OF ENVIRONMENTAL SCIENCES 34(2015):143-154.