Re-evaluation of thyroid hormone signaling antagonism of tetrabromobisphenol A for validating the T3-induced Xenopus metamorphosis assay

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Re-evaluation of thyroid hormone signaling antagonism of tetrabromobisphenol A for validating the T3-induced Xenopus metamorphosis assay
	Wang, Yao; Li, Yuanyuan; Qin, Zhanfen; Wei, Wuji
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2017-07-01
卷号	52 期号:0 页码:325-332
关键词	Thyroid Hormone Signaling Tetrabromobisphenol a Xenopus Metamorphosis T3 Induction Tail
英文摘要	We developed the T3-induced Xenopus metamorphosis assay, which is supposed to be able to sensitively detect thyroid hormone (TH) signaling disruption of chemicals. The present study aimed to validate the T3-induced Xenopus metamorphosis assay by re-evaluating the TH signaling antagonism of tetrabromobisphenol A (TBBPA), a known TH signaling disruptor. According to the assay we developed, Xenopus tadpoles at stage 52 were exposed to 10-500 nmol/L TBBPA in the presence of 1 nmol/L T3. After 96 hr of exposure, TBBPA in the range of 10-500 nmol/L was found to significantly inhibit T3-induced morphological changes of Xenopus tadpoles in a concentration-dependent manner in term of body weight and four morphological endpoints including head area (HA), mouth width (MW), unilateral brain width/brain length (ULBW/BL), and hind-limb length/snout-vent length (HLL/SVL). The results show that these endpoints we developed are sensitive for characterizing the antagonistic effects of TBBPA on T3-induced metamorphosis. Following a 24-hr exposure, we found that TBBPA antagonized expression of T3-induced TH-response genes in the tail, which is consistent with previous findings in the intestine. We propose that the tail can be used as an alternative tissue to the intestine for examining molecular endpoints for evaluating TH signaling disruption. In conclusion, our results demonstrate that the T3-induced Xenopus metamorphosis assay we developed is an ideal in vivo assay for detecting TH signaling disruption. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39402]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Yao,Li, Yuanyuan,Qin, Zhanfen,et al. Re-evaluation of thyroid hormone signaling antagonism of tetrabromobisphenol A for validating the T3-induced Xenopus metamorphosis assay[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2017,52(0):325-332.
APA	Wang, Yao,Li, Yuanyuan,Qin, Zhanfen,&Wei, Wuji.(2017).Re-evaluation of thyroid hormone signaling antagonism of tetrabromobisphenol A for validating the T3-induced Xenopus metamorphosis assay.JOURNAL OF ENVIRONMENTAL SCIENCES,52(0),325-332.
MLA	Wang, Yao,et al."Re-evaluation of thyroid hormone signaling antagonism of tetrabromobisphenol A for validating the T3-induced Xenopus metamorphosis assay".JOURNAL OF ENVIRONMENTAL SCIENCES 52.0(2017):325-332.