Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer
	Liu, Yingxiong; Zhao, Qiang
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2017-07-14
卷号	409 期号:16 页码:3993-4000
关键词	Aptamer Fluorescence Anisotropy Fluorescence Polarization Cocaine Small Molecule Sensors
英文摘要	Development of simple, sensitive, and rapid method for cocaine detection is important in medicine and drug abuse monitoring. Taking advantage of fluorescence anisotropy and aptamer, this study reports a direct fluorescence anisotropy (FA) assay for cocaine by employing an aptamer probe with tetramethylrhodamine (TMR) labeled on a specific position. The binding of cocaine and the aptamer causes a structure change of the TMR-labeled aptamer, leading to changes of the interaction between labeled TMR and adjacent G bases in aptamer sequence, so FA of TMR varies with increasing of cocaine. After screening different labeling positions of the aptamer, including thymine (T) bases and terminals of the aptamer, we obtained a favorable aptamer probe with TMR labeled on the 25th base T in the sequence, which exhibited sensitive and significant FA-decreasing responses upon cocaine. Under optimized assay conditions, this TMR-labeled aptamer allowed for direct FA detection of cocaine as low as 5 mu M. The maximum FA change reached about 0.086. This FA method also enabled the detection of cocaine spiked in diluted serum and urine samples, showing potential for applications.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39295]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Yingxiong,Zhao, Qiang. Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(16):3993-4000.
APA	Liu, Yingxiong,&Zhao, Qiang.(2017).Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(16),3993-4000.
MLA	Liu, Yingxiong,et al."Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.16(2017):3993-4000.