A Rapid and Ultrasensitive Tetraphenylethylene-Based Probe with Aggregation-Induced Emission for Direct Detection of alpha-Amylase in Human Body Fluids

doi:10.1021/acs.analchem.8b04244

	A Rapid and Ultrasensitive Tetraphenylethylene-Based Probe with Aggregation-Induced Emission for Direct Detection of alpha-Amylase in Human Body Fluids
	Tang, Bo 3; Huang, Fenghong 4; Li, Lu 3; Zheng, Zhe 1; Wan, Chuyun 4; Chai, Zhaofei 2; Zheng, Mingming 4; Li, Yu 4; Deng, Qianchun 4; Shi, Jie 3,4
刊名	ANALYTICAL CHEMISTRY
	2018-11-20
卷号	90 期号:22 页码:13775-13782
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b04244
英文摘要	alpha-Amylase plays a key role in the physiological cycle of the human body; its function is constantly explored and used as an important indicator of some related diseases like acute pancreatitis, acute organophosphorus pesticide poisoning, and anxiety or depression. However, currently, including the assay kit, existing methods suffer from low sensitivity and time consumption or are indirect assays that require the aid of a tool enzyme or inhibitor of competitive substrates; hence, they are not suitable for the low activity and nondestructive sensing of alpha-amylase in body fluids. A rapid, highly sensitive, and simple direct alpha-amylase determination in human body fluids is still challenging. In this work, an AlEgen-based small molecule a-amylase sensing system was first established. The probe has no emission signal in aqueous media because of its good solubility, but the insoluble AIE residues can be released after hydrolysis by alpha-amylase, lighting up fluorescence significantly. In this novel sensing system, the detection limit is calculated to be 0.14 U L-1 in MES buffer with a linear range of 0-45.5 U L-1, having been shortened to 3 min of test time and excellent selectivity to alpha-amylase compared to other proteins. Moreover, our method is successfully employed to demonstrate the applications in acute pancreatitis diagnosis and psychological stress analysis. The acquisition of this ME-based method not only provides a simple technique for clinical diagnosis of related diseases but also has a promotional value for the food and pharmaceutical industries.
资助项目	National Science Foundation of China[31872896] ; National Science Foundation of China[21535004] ; National Science Foundation of China[91753111] ; National Science Foundation of China[31501423] ; National Science Foundation of China[21390411] ; Young Elite Scientists Sponsorship Program by CAST[2017QNRC001] ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences[CAAS-ASTIP-2013-OCRI]
WOS关键词	ACUTE-PANCREATITIS ; SERUM ; BIOMARKERS ; STRESS ; STARCH ; AIEGEN ; STATE ; ASSAY
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000451246100085
资助机构	National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13535]
专题	中国科学院武汉物理与数学研究所
通讯作者	Tang, Bo; Huang, Fenghong
作者单位	1.Univ Sci & Technol China, Dept Modern Phys, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 3.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Shandong, Peoples R China 4.Chinese Acad Agr Sci, Hubei Key Lab Lipid Chem & Nutr, Key Lab Oilseeds Proc, Minist Agr,Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Tang, Bo,Huang, Fenghong,Li, Lu,et al. A Rapid and Ultrasensitive Tetraphenylethylene-Based Probe with Aggregation-Induced Emission for Direct Detection of alpha-Amylase in Human Body Fluids[J]. ANALYTICAL CHEMISTRY,2018,90(22):13775-13782.
APA	Tang, Bo.,Huang, Fenghong.,Li, Lu.,Zheng, Zhe.,Wan, Chuyun.,...&Shi, Jie.(2018).A Rapid and Ultrasensitive Tetraphenylethylene-Based Probe with Aggregation-Induced Emission for Direct Detection of alpha-Amylase in Human Body Fluids.ANALYTICAL CHEMISTRY,90(22),13775-13782.
MLA	Tang, Bo,et al."A Rapid and Ultrasensitive Tetraphenylethylene-Based Probe with Aggregation-Induced Emission for Direct Detection of alpha-Amylase in Human Body Fluids".ANALYTICAL CHEMISTRY 90.22(2018):13775-13782.