Molecular Characterization of Thyroid Hormone Receptors (TRs) and their Responsiveness to T3 in Microhyla fissipes

doi:10.16373/j.cnki.ahr.170091

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 《亚洲两栖爬行动物研究》 > 2018 > Vol.9, No.1

	Molecular Characterization of Thyroid Hormone Receptors (TRs) and their Responsiveness to T3 in Microhyla fissipes
	Jianping JIANG; Mengjie ZHANG; Xungang WANG; Lusha LIU
刊名	Asian Herpetological Research
	2018-03
卷号	9 期号:1 页码:13-23
关键词	Microhyla fissipes thyroid hormone receptors functional characteristic expression pattern responsiveness
DOI	10.16373/j.cnki.ahr.170091
英文摘要	To explore and enrich the molecular mechanisms of thyroid hormone receptors (TRs) in the metamorphosis of amphibians, the cDNA sequences of TRα and TRβ in Microhyla fissipes were cloned and characterized. TRα was 1 706 bp in length with an open reading frame (ORF) of 1 257 bp encoding a predicted protein of 418 amino acids and TRβ was 1 422 bp with an ORF of 1 122 bp encoding a predicted protein of 373 amino acids. Their protein sequences contained 4 conserved domains of the nuclear receptor superfamily with two highly conserved cysteine-rich zinc fingers in the DNA-binding domain, whereas TRβ was 42 amino acids shorter in its A/B domain than TRα. Highly-conserved sequences and structures indicated their conserved functions during metamorphosis. TRα expression reached peak at 12 h and then decreased from 12 h to 48 h. While dramatically up-regulated TRβ was observed after exposure of T3 within 24 h, and it was down-regulated from 24 h to 48 h. The expression pattern of TRβ is similar to that in the natural metamorphosis. Furthermore, tadpoles treated 24 h also resembled the climax of metamorphosis tadpoles and TRβ expression had higher responsiveness than TRα to T3 in M. fissipes. These results suggest M. fissipes may serve as the model to assay environmental compounds on TH signaling disruption.
语种	英语
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29575]
专题	《亚洲两栖爬行动物研究》_2018_Vol.9, No.1
推荐引用方式 GB/T 7714	Jianping JIANG,Mengjie ZHANG,Xungang WANG,et al. Molecular Characterization of Thyroid Hormone Receptors (TRs) and their Responsiveness to T3 in Microhyla fissipes[J]. Asian Herpetological Research ,2018,9(1):13-23.
APA	Jianping JIANG,Mengjie ZHANG,Xungang WANG,&Lusha LIU.(2018).Molecular Characterization of Thyroid Hormone Receptors (TRs) and their Responsiveness to T3 in Microhyla fissipes.Asian Herpetological Research ,9(1),13-23.
MLA	Jianping JIANG,et al."Molecular Characterization of Thyroid Hormone Receptors (TRs) and their Responsiveness to T3 in Microhyla fissipes".Asian Herpetological Research 9.1(2018):13-23.