| 题名 | 白桦响应盐胁迫的生理学机制及相关基因的鉴定 |
| 作者 | 美合日古丽·米吉提 |
| 答辩日期 | 2017-05-01 |
| 文献子类 | 硕士 |
| 授予单位 | 中国科学院大学 |
| 授予地点 | 新疆乌鲁木齐 |
| 导师 | 王玉成 |
| 关键词 | 白桦 生理学响应 LEA 基因 盐胁迫 qRT-PCR Betula platyhylla Suk physiological responses LEA gene salt stress qRT-PCR |
| 学位专业 | 工程硕士 |
| 英文摘要 | Birches belong to the genus Betula in the family Betulaceae. Birch trees arefast-growing woody plants that adapt to adverse environments, and are widely distributedfrom north Europe to Russia, north of China, and Japan. Therefore, it is important tostudy the mechanism of salt stress tolerance in birch plants. Recently, the sequencingwhole-genome of Betula platyphylla Suk. has been completed. Therefore, it has laid asolid foundation for studying the Betula platyphylla Suk’s genetic resources. Here, we treated birch seedlings with a solution of 100 mM NaCl and studied thephysiological and related molecular response of white birch (Betula Platyphylla Suk.) tosalt stress. We found that abscisic acid and Ca2+level were induced in birch during theearly salt stress period, ABA was induced during early salt stress period, and thendecreased, and Ca2+level increased slowly but maintained at a high level for a long time. Under salt conditions, photosynthetic activity was inhibited; the Salt-Overly-Sensitive(SOS) pathway was activated in birch roots; reactive oxygen species (ROS) wasaccumulated, and superoxide dismutase is the main ROS scavenger in roots, whileperoxidase is the main ROS scavenger in leaves. Proline plays a role in stress tolerance inboth roots and leaves; however, soluble sugars and trehalose also have roles in salt stresstolerance, mainly in leaves. Additionally, the genes that might have essential roles incontrolling some of these physiological changes were identified. In leaves, the POD5 andPOD8 genes might play a main role in increase the POD activity. In roots, the SOD1 andSOD4 genes might make main contributions to the increased SOD activity during saltstress. P5CS1 is closely related to the biosynthesis of proline in roots, and P5CS2 mightplay a main role in proline biosynthesis in leaves; the TPS4 and TPP3 genes might playimportant roles in the biosynthesis of trehalose in birch during salt stress period. Therefore, they represent good candidate genes to characterize the salt tolerancemechanism of birch and to breed stress tolerant plants. Late Embryogenesis Abundant (LEA) proteins are accumulated during the last period of seed development, and also play important roles in abiotic stress tolerance of plants. Inthe present study, we studied the 13 LEA genes that are highly similar with the stresstolerance LEA genes in Arabidopsis, and they were cloned from B. platyphylla. Inaddition, the LEA genes that are in response to salt stress were further identified usingqRT-PCR. These LEA genes involving in salt stress response will be the good candidategenes in genetic approaches for breeding salt stress tolerance in plants. |
| 语种 | 中文 |
| 学科主题 | 生物工程 |
| 内容类型 | 学位论文 |
| 源URL | [http://ir.xjlas.org/handle/365004/14892]  |
| 专题 | 新疆生态与地理研究所_研究系统_荒漠环境研究室 |
| 作者单位 | 中国科学院新疆生态与地理研究所 |
| 推荐引用方式 GB/T 7714 | 美合日古丽·米吉提. 白桦响应盐胁迫的生理学机制及相关基因的鉴定[D]. 新疆乌鲁木齐. 中国科学院大学. 2017. |
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