Probabilistic Modeling of Rosette Formation

CORC > 力学研究所 > 中国科学院力学研究所 > 力学所知识产出(1956-2008)

	Probabilistic Modeling of Rosette Formation
	Long M(龙勉); Chen J(陈娟); Jiang N(姜宁); Selvaraj P; McEver RP; Zhu C(朱承)
刊名	Biophysical Journal
	2006
通讯作者邮箱	mlong@imech.ac.cn
卷号	91 期号:1 页码:352-363
ISSN号	0006-3495
通讯作者	Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
中文摘要	英文摘要: Rosetting, or forming a cell aggregate between a single target nucleated cell and a number of red blood cells (RBCs), is a simple assay for cell adhesion-mediated by specific receptor-ligand interaction. For example, rosette formation between sheep RBC and human lymphocytes has been used to differentiate T cells from B cells. Rosetting assay is commonly used to determine the interaction of Fc gamma-receptors (Fc gamma R) expressed on inflammatory cells and IgG-coated on RBCs. Despite its wide use in measuring cell adhesion, the biophysical parameters of rosette formation have not been well characterized. Here we developed a probabilistic model to describe the distribution of rosette sizes, which is Poissonian. The average rosette size is predicted to be proportional to the apparent two-dimensional binding affinity of the interacting receptor-ligand pair and their site densities. The model has been supported by experiments of rosettes mediated by four molecular interactions: Fc gamma RIII interacting with IgG, T cell receptor and coreceptor CD8 interacting with antigen peptide presented by major histocompatibility molecule, P-selectin interacting with P-selectin glycoprotein ligand 1 (PSGL-1), and L-selectin interacting with PSGL-1. The latter two are structurally similar and are different from the former two. Fitting the model to data enabled us to evaluate the apparent effective two-dimensional binding affinity of the interacting molecular pairs: 7.19x10(-5) mu m(4) for Fc gamma RIII-IgG interaction, 4.66x10(-3) mu m(4) for P-selectin-PSGL-1 interaction, and 0.94x10(-3) mu m(4) for L-selectin-PSGL-1 interaction. These results elucidate the biophysical mechanism of rosette formation and enable it to become a semiquantitative assay that relates the rosette size to the effective affinity for receptor-ligand binding.
学科主题	力学
类目[WOS]	Biophysics
研究领域[WOS]	Biophysics
关键词[WOS]	SELECTIN GLYCOPROTEIN LIGAND-1 ; CELL-ADHESION ; P-SELECTIN ; CONCURRENT BINDING ; KINETIC RATES ; RECEPTOR ; AFFINITY ; IDENTIFICATION ; ERYTHROCYTES ; PEPTIDE
收录类别	SCI
语种	英语
WOS记录号	WOS:000238288200035
公开日期	2007-06-15 ; 2007-12-05 ; 2009-06-23
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/17673]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Long M,Chen J,Jiang N,et al. Probabilistic Modeling of Rosette Formation[J]. Biophysical Journal,2006,91(1):352-363.
APA	龙勉,陈娟,姜宁,Selvaraj P,McEver RP,&朱承.(2006).Probabilistic Modeling of Rosette Formation.Biophysical Journal,91(1),352-363.
MLA	龙勉,et al."Probabilistic Modeling of Rosette Formation".Biophysical Journal 91.1(2006):352-363.