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	Cultivation of two species of Marine Microalgae for Targets Accumulation; 两种海洋微藻培养积累目标产物
	ChanghongYao、DinaFeng、ZhaoanChen、SongXue
	2010-07-23
会议名称	2010 internationnal symposium on advanced biological engineeringi
会议日期	2010-7-23
会议地点	中国
其他题名	两种海洋微藻培养积累目标产物
页码	68/2
通讯作者	薛松
中文摘要	starch constitutes a major carbon sink for co2 fixation during the process of photosynthesis of microalgae. some microalgae, primary green algae, have been proved to accumulate large amount of intracellular starch under stress conditions. it is strikingly valuable and important for production of algal-derived starch in large scale to meet the demand for renewable and clean energy (bioethanol, bio-hydrogen, etc.). a marine green microalga tetraselmis subcordiformis was investigated its growth as well as intracellular starch accumulation in a 10l flat airlift photobioreactor (420×80×500 mm) with inside channel under continuous illumination (120~250 μ mol e • m-2 • s-1) at 30℃. 3% co2- rich air was compressed into the photobioreactor to reach the aeration of 0.125 vvm. the results showed that under nitrogen limiting condition (1.18 mmol/l initial no3-), t. subcordiformis was able to accumulate starch from initially 20% (dry weight based) to more than 50% within five days. the decline of chlorophyll florescence dynamics parameters might be attributed to the starch accumulation under the photosynthetic stressed condition. microalgae lipids, as a source of renewable biofuels, have attracted more and more attentions due to their ability to produce biodiesel and hydrocarbon gas [5]. in order to improve the lipid content, the most effective culture condition was the depletion of the major nutrient nitrogen [6]. in our laboratory, we studied the supplement time of sodium nitrate and the effects of different nitrogen sources on cell growth and lipid accumulation of isochrysis. zhangjiangensis. using 0.6 l tubular photobioreactors (diameter5cm×height 70cm), 882µmol.l-1sodium nitrate was supplied in f/2 culture medium for 24h once, 48h once, 72h once, respectively. the initial cell density was 8×106cell.ml-1. the experiments were performed at 25±1ºc, 2%co2 supply of 0.8 vvm and light/dark 14:10 at light intensity of 200µem−2s−1. the highest biomass of 2.4± 0.6g.l-1was obtained when the medium was added every day. the highest total lipid content 44.9 ± 0.1% and lipid productivity 119.7 ± 29.9 mg.l-1day-1 were achieved when nitrogen source is supplied every day. the variation in fatty acid composition of three cultures is significant and sixteen kinds of fatty acids are monitored in the cultures for. the highest biomass productivity was obtained when 882µmol.l-1urea was supplied once a day, with a biomass concentration of 3.09±0.27 g/l and a lipid content of 36.1±1.5%.
会议主办者	清华大学和日本东京工业大学
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/114108]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	ChanghongYao、DinaFeng、ZhaoanChen、SongXue. Cultivation of two species of Marine Microalgae for Targets Accumulation, 两种海洋微藻培养积累目标产物[C]. 见:2010 internationnal symposium on advanced biological engineeringi. 中国. 2010-7-23.

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