A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection

doi:10.1371/journal.pone.0198102

	A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection
	Qian, Chunqi 1; Suo, Yourui2 ; You, Jinmao2 ; Fan, Baolei 2,3
刊名	PLOS ONE
	2018-06-06
卷号	13 期号:6
ISSN号	1932-6203
DOI	10.1371/journal.pone.0198102
文献子类	Article
英文摘要	A new labeling reagent for vitamin analysis, 2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The reaction of AEAO with vitamins could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as condensing reagent within 45 min. The derivatives exhibited excellent fluorescence property with excitation and emission wavelengths of 290 nm and 430 nm, respectively. Response surface methodology (RSM) was applied to the optimization of pre-column derivatization. Solid phase extraction with HLB cartridges was used for the extraction and purification of water-soluble vitamins in tea samples. The LODs for vitamin B3 and vitamin B7 were 2.56 and 2.22 ng mL(-1), respectively. The proposed method was successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The study provided a highly sensitive method for accurate analysis of trace vitamins from natural products.
WOS关键词	WATER-SOLUBLE VITAMINS ; MASS-SPECTROMETRY ; ENRICHED DRINK ; MULTIVITAMIN ; TAURINE ; TABLETS ; INGREDIENTS ; RIBOFLAVIN ; METABOLISM ; PYRIDOXINE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000434369400039
资助机构	Educational Commission of Hubei Province of China(D20142806) ; Plan Projects of Science and Technology of Hubei Province of China(2015CFC777)
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13806]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
通讯作者	Qian, Chunqi; Suo, Yourui
作者单位	1.Michigan State Univ, E Lansing, MI 48824 USA 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China 3.Hubei Univ Sci & Technol, Xianning, Peoples R China
推荐引用方式 GB/T 7714	Qian, Chunqi,Suo, Yourui,You, Jinmao,et al. A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection[J]. PLOS ONE,2018,13(6).
APA	Qian, Chunqi,Suo, Yourui,You, Jinmao,&Fan, Baolei.(2018).A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection.PLOS ONE,13(6).
MLA	Qian, Chunqi,et al."A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection".PLOS ONE 13.6(2018).