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	Expression profiling of key genes involved in taxuyunnanine C biosynthesis in suspension cultures of Taxus chinensis by sucrose feeding and double elicitation with 2,3-dihydroxylpropanyl jasmonate
	Gao MB(高明波) ; Zhang W(张卫) ; Jin MF(金美芳)
	2008-10-12
会议名称	13th international biotechnology symposium and exhibition
会议日期	2008-10-12
会议地点	中国
页码	s155/2
通讯作者	张卫
中文摘要	taxus suspension cell culture has the potential to provide a sustainable source of anticancer drug paclitaxel (taxol®) and other taxoids. in the cell culture of taxus chinensis, taxuyunnanine c (tc) is the primary taxoid. to design a rational strategy for redirecting the precursor fluxes from other taxoids into paclitaxel production, this study employed quantitative rt-pcr to understand the dynamic profiling of key biosynthetic pathway genes of palcitaxel and taxoids during the cultureprocess. six genes (tasy,tdat, t5 h, t h, tdat and t10 h) were quantified under a process condition of double elicitation by 2,3-dihydroxylpropanyl jasmonate(dhpja) (100 m) on days 7 and 12, and sucrose feeding (20 g/l) on day 7. this process treatment led to a high accumulation of taxuyunnanine c (tc) at 554.46±21.28 mg/l 8 days after the first elicitation, 5.5-fold of that of control. and 9 days after the second elicitation, tc production was as high as 997.72±1.51 mg/l, 9.7-fold of that of control. the early pathway genes tasy and tdat were signifi- cantly up-regulated by 182-fold and 98-fold, respectively for the first dhpja elicitation and by 208-fold and 131-fold, respectively for the second elicitation. the induction after each elicitation lasted for about 24 h before their abundances decreased. things are some different in the case of the other four genes t5 h, t h, tdat and t10 h. for gene t h, it was greatly up-regulated by 3061-fold for the first dhpja elicitation and by 1016-fold for the second elicitation. for the other three genes t5 h, t10 h, t14 h, they were up-regulated by 13-fold, 38-fold and 20-fold, respectively for the firstdhpja elicitation and by 7-fold, 16-fold and 6-fold, respectively for the second elicitation.
会议主办者	中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
学科主题	物理化学
语种	中文
WOS记录号	WOS:000208979400350
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113102]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Gao MB,Zhang W,Jin MF. Expression profiling of key genes involved in taxuyunnanine C biosynthesis in suspension cultures of Taxus chinensis by sucrose feeding and double elicitation with 2,3-dihydroxylpropanyl jasmonate[C]. 见:13th international biotechnology symposium and exhibition. 中国. 2008-10-12.

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