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	Carbonylcyanide m-chlorophenylhrazone (CCCP) regulated hydrogen production by marine green algae Platymonas subcordiformis subjected to light-dark-light treatment
	Fu YB(傅赟彬) ; Chen ZA(陈兆安) ; Guo Z(郭祯) ; Lu HB(陆洪斌) ; Yu XJ(虞星炬) ; Zhang W(张卫)
	2008-10-12
会议名称	13th international biotechnology symposium and exhibition
会议日期	2008-10-12
会议地点	中国
页码	s563/2
通讯作者	张卫
中文摘要	platymonas subcordiformiswas found to produce hydrogen via photolysis of seawater (guan et al., 2004). in order to improve hydrogen production, the effect of light-dark-light treatment on the duration and yield of hydrogen evolutionwas investigated in the process reg ulated by cccp. cccpwas found to inhibit the photosystem ii (ps ii) activity, therefore creating an anaerobic environment essential for the onset of hydrogen evolution (ran et al., 2006). in our system, a fuel-cell operated by hydrogenwas integrated into the photobioreactor for both on-line monitoring of the rate of hydrogen evolution and the reduction of potential hydrogen feedback inhibition by consuming hydrogen in situ. during the first 15 h of hydrogen evolution stage, a period of dark treatment followed the initial light illumination with three testing regimes (9 h/6 h; 6 h/9 h; 3 h/12 h) were investigated, with continuous illumination as the control. after the dark treatment, all the cultures were subjected to continuous light illumination until the hydrogen evolution stopped. three peaks of hydrogen evolution rate were found under all three lightdark- light treatments, in contrast to only one peak rate observed under continuous illumination. in addition, hydrogen production duration was extended from 40 h under continuous illumination to 120 h. the highest hydrogen yield of 78 ml/l achieved using a light-dark-light treatment of 9 h/6 h/ to the end,was similar to that of continuous illumination. these results indicate that the diurnal alternation of solar energy may be suitable for hydrogen production by marine green algae without sacrificing hydrogen yield, and that artificial night-time illumination may be unnecessary to total cell productivity.
会议主办者	中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
学科主题	物理化学
语种	中文
WOS记录号	WOS:000208979402283
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113074]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Fu YB,Chen ZA,Guo Z,et al. Carbonylcyanide m-chlorophenylhrazone (CCCP) regulated hydrogen production by marine green algae Platymonas subcordiformis subjected to light-dark-light treatment[C]. 见:13th international biotechnology symposium and exhibition. 中国. 2008-10-12.

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