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	Development and application of sol-gel immunoaffinity extraction-based methods for the determination of diuron in surface water
	Zhang XL(张秀莉) ; DieterMartens ; PetraM.Krä ; mer ; AntoniusA.Kettrup ; Liang XM(梁鑫淼)
	2007-06-04
会议名称	dalian international symposia and exhibition on chromatography
会议日期	2007-6-4
会议地点	中国
页码	25/2
通讯作者	梁鑫淼
中文摘要	an anti-diuron immunoaffinity extraction column was fabricated for the first time by encapsulation of anti-diuron monoclonal antibodies in sol-gel glass matrix. the experimental capacity of the sol-gel immunoaffinity column (sg-iac) immobilized 240 g antibodies was 302.1±11.6 ng (n=4) of diuron, corresponding to 40% of the theoretical maximum capacity. the antibody density in sg-iac up to 3.75 mg g-1 resulted the linear increasing of experimental capacity. the immunoaffinity column-based loading, rinsing and eluting processes were optimized. based on these optimizations, the sg-iac selectively extracted diuron from an artificial mixture of 64 pesticides. in addition to this high selectivity, the sg-iac proved to be reusable. the sg-iac was combined with liquid chromatography-tandem mass spectrometry to determine diuron in surface water, and the linear range was up to 2.2 g l-1 with correlation coefficient higher than 0.99 and relative standard deviation lower than 5.7% (n=8). the limit of quantitation for 25-ml surface water sample was 3.9 ng l-1.
会议主办者	中国科学院大连化学物理研究所
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/112500]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Zhang XL,DieterMartens,PetraM.Krä,et al. Development and application of sol-gel immunoaffinity extraction-based methods for the determination of diuron in surface water[C]. 见:dalian international symposia and exhibition on chromatography. 中国. 2007-6-4.

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