| Genome editing for cancer therapy: delivery of cas9 protein/sgrna plasmid via a gold nanocluster/lipid core-shell nanocarrier | |
| Wang, Peng1; Zhang, Lingmin1; Xie, Yangzhouyun1; Wang, Nuoxin1; Tang, Rongbing1; Zheng, Wenfu1; Jiang, Xingyu1,2 | |
| 刊名 | Advanced science
 |
| 2017-11-01 | |
| 卷号 | 4期号:11页码:10 |
| 关键词 | Cancer therapy Crispr/cas9 Genome editing Gold nanoclusters |
| ISSN号 | 2198-3844 |
| DOI | 10.1002/advs.201700175 |
| 通讯作者 | Zheng, wenfu(zhengwf@nanoctr.cn) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | The type ii bacterial clustered, regularly interspaced, short palindromic repeats (crispr)-cas9 (crispr-associated protein) system (crispr-cas9) is a powerful toolbox for gene-editing, however, the nonviral delivery of crispr-cas9 to cells or tissues remains a key challenge. this paper reports a strategy to deliver cas9 protein and single guide rna (sgrna) plasmid by a nanocarrier with a core of gold nanoclusters (gns) and a shell of lipids. by modifying the gns with hiv-1-transactivator of transcription peptide, the cargo (cas9/sgrna) can be delivered into cell nuclei. this strategy is utilized to treat melanoma by designing sgrna targeting polo-like kinase-1 (plk1) of the tumor. the nanoparticle (polyethylene glycol-lipid/gns/cas9 protein/sgplk1 plasmid, lgcp) leads to >70% down-regulation of plk1 protein expression of a375 cells in vitro. moreover, the lgcp suppresses melanoma progress by 75% on mice. thus, this strategy can deliver protein-nucleic acid hybrid agents for gene therapy. |
| WOS关键词 | EFFICIENT DELIVERY ; MEDIATED DELIVERY ; SIRNA DELIVERY ; MESSENGER-RNA ; HUMAN-CELLS ; NANOPARTICLES ; CRISPR-CAS9 ; DNA ; SYSTEM |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000416155900007 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2177793 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Zheng, Wenfu; Jiang, Xingyu |
| 作者单位 | 1.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sinodanish Coll, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Peng,Zhang, Lingmin,Xie, Yangzhouyun,et al. Genome editing for cancer therapy: delivery of cas9 protein/sgrna plasmid via a gold nanocluster/lipid core-shell nanocarrier[J]. Advanced science,2017,4(11):10. |
| APA | Wang, Peng.,Zhang, Lingmin.,Xie, Yangzhouyun.,Wang, Nuoxin.,Tang, Rongbing.,...&Jiang, Xingyu.(2017).Genome editing for cancer therapy: delivery of cas9 protein/sgrna plasmid via a gold nanocluster/lipid core-shell nanocarrier.Advanced science,4(11),10. |
| MLA | Wang, Peng,et al."Genome editing for cancer therapy: delivery of cas9 protein/sgrna plasmid via a gold nanocluster/lipid core-shell nanocarrier".Advanced science 4.11(2017):10. |
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