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	An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers
	Hu, Binfeng1,2; Liu, Yong1,2; Deng, Jinqi1,2; Mou, Lei1,2; Jiang, Xingyu1,2
刊名	Analytical methods
	2018-06-07
卷号	10期号:21页码:2470-2480
ISSN号	1759-9660
DOI	10.1039/c8ay00682b
通讯作者	Jiang, xingyu(xingyujiang@nanoctr.cn)
英文摘要	In this study, we developed a microfluidic chip integrated with on-chip valves for quantitative detection of biomarkers. the sandwich-structured microfluidic chip consists of a top fluidic layer embedded with a zigzag channel, a middle tinfoil layer pre-patterned with protein stripes, and a bottom substrate layer with two waste chambers. a set of customized on-chip valves was introduced for precise control of fluid flow. to improve the production volume and uniformity among chips, we selected an injection molding method for mass-production of the main components. we standardized the process of sealing chip layers and assembling the valve holder to improve the effectiveness of cooperation with the instrument. to demonstrate the versatility of the microfluidic chip for quantitative detection of biomarkers, we used it to automatically detect c-reactive proteins (crp), interleukin-6 (il-6), insulin, and osteocalcin with high sensitivity and excellent reproducibility inside a customized and portable instrument. we further conducted the automated chip-based chemiluminescence immunoassay to test crp in serum samples for demonstration of potential clinical applications, assess the activity of capture antibodies of crp (crp-ab(1)) on the tinfoil layer for preliminary assessment of storage stability, and simultaneously detect crp and il-6 for confirmation of scalable multiplexation. this microfluidic chip for quantitative, automated, and portable immunoassay provides a promising choice for clinical practices.
WOS关键词	CHEMILUMINESCENCE IMMUNOASSAY ; PERFORMANCE EVALUATION ; AMPLIFICATION ; DEVICES ; CELLS ; QUANTIFICATION ; MICROVALVES ; EMBRYOS ; VIRUSES ; PUMPS
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目	Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000434311500007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2177536
专题	高能物理研究所
通讯作者	Jiang, Xingyu
作者单位	1.Natl Ctr Nanosci & Technol, Beijing Engn Res Ctr BioNanotechnol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Binfeng,Liu, Yong,Deng, Jinqi,et al. An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers[J]. Analytical methods,2018,10(21):2470-2480.
APA	Hu, Binfeng,Liu, Yong,Deng, Jinqi,Mou, Lei,&Jiang, Xingyu.(2018).An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers.Analytical methods,10(21),2470-2480.
MLA	Hu, Binfeng,et al."An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers".Analytical methods 10.21(2018):2470-2480.

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