| Lal test and rpt for endotoxin detection of cpt-11/dspe-mpeg(2000) nanoformulation: what if traditional methods are not applicable? | |
| Jin, Yanan1; Jia, Juanjuan2; Li, Chan3,4; Xue, Jianqi5; Sun, Jiabei2; Wang, Kaiyuan3,4; Gan, Yaling3,4; Xu, Jing3,4; Shi, Yaqin2; Liang, Xingjie1,3,4,6 | |
| 刊名 | Asian journal of pharmaceutical sciences
 |
| 2018-05-01 | |
| 卷号 | 13期号:3页码:289-296 |
| 关键词 | Irinotecan hydrochloride Endotoxin detection Micelle Dspe-mpeg(2000) Limulus amebocyte lysate assay Rabbit pyrogen test |
| ISSN号 | 1818-0876 |
| DOI | 10.1016/j.ajps.2017.11.003 |
| 通讯作者 | Xu, jing(xujing@nanoctr.cn) ; Shi, yaqin(shiyq@nifdc.org.cn) ; Liang, xingjie(liangxj@nanoctr.cn) |
| 英文摘要 | Endotoxin detection is an important step in drug characterization. herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride (cpt-11) and an amphiphilic molecule dspe-mpeg(2000) can interfere with the limulus amebocyte lysate assay (lal). furthermore, the rabbit pyrogen test (rpt) results indicated that at a relatively high dosage, the drug irinotecan hydrochloride can induce a hypothermia effect which may render the rpt results ambiguous in determination of the safety of the drug formulation. our findings demonstrate limitations of endotoxin detection in micellar drugs, and call for the necessity of developing reliable endotoxin detection methods that can overcome the interference of nanomaterials in order to better ensure the drug safety of patients in future pharmaceutical drug development. (c) 2018 shenyang pharmaceutical university. production and hosting by elsevier b.v. |
| WOS关键词 | NANOPARTICLES ; CHALLENGES |
| WOS研究方向 | Pharmacology & Pharmacy |
| WOS类目 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | HONG KONG ASIAMED PUBLISH HOUSE |
| WOS记录号 | WOS:000432445400010 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2177503 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Xu, Jing; Shi, Yaqin; Liang, Xingjie |
| 作者单位 | 1.Hebei Univ, Coll Chem & Environm Sci, Key Lab Med Chem & Mol Diag, Minist Educ, Baoding 071002, Peoples R China 2.Natl Inst Food & Drug Control, 5 Huatuo Rd, Beijing 102629, Peoples R China 3.Chinese Acad Sci CAS Ctr Excellence Nanosci, Lab Controllable Nanopharmaceut, 11 First North Rd Zhongguancun, Beijing 100190, Peoples R China 4.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, 11 First North Rd Zhongguancun, Beijing 100190, Peoples R China 5.Hebei Normal Univ, Coll Life Sci, Lab Mol Iron Metab, Shijiazhuang 050024, Hebei, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Yanan,Jia, Juanjuan,Li, Chan,et al. Lal test and rpt for endotoxin detection of cpt-11/dspe-mpeg(2000) nanoformulation: what if traditional methods are not applicable?[J]. Asian journal of pharmaceutical sciences,2018,13(3):289-296. |
| APA | Jin, Yanan.,Jia, Juanjuan.,Li, Chan.,Xue, Jianqi.,Sun, Jiabei.,...&Liang, Xingjie.(2018).Lal test and rpt for endotoxin detection of cpt-11/dspe-mpeg(2000) nanoformulation: what if traditional methods are not applicable?.Asian journal of pharmaceutical sciences,13(3),289-296. |
| MLA | Jin, Yanan,et al."Lal test and rpt for endotoxin detection of cpt-11/dspe-mpeg(2000) nanoformulation: what if traditional methods are not applicable?".Asian journal of pharmaceutical sciences 13.3(2018):289-296. |
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