| Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines | |
| Sun, Hai-Yuan1; Wu, Fu-Gen2; Li, Zhi-Hong3; Deng, Geng1; Zhou, Yu1,4; Yu, Zhi-Wu1 | |
| 刊名 | Rsc advances
 |
| 2017 | |
| 卷号 | 7期号:10页码:5715-5724 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c6ra24961b |
| 通讯作者 | Yu, zhi-wu(yuzhw@tsinghua.edu.cn) |
| 英文摘要 | Bilayered micelles, or so-called bicelles, are generally made of long-and short-chain lipids. they are extensively used as model membranes to study the structure of membrane-associated peptides or proteins and their interactions with membranes. however, the phase behavior of lipid mixtures composed of longand short-chain lipids, especially at low temperatures, is still not very clear. in this work, the most commonly used long-chain lipid, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (dppc), and a short-chain lipid, 1,2-dioctanoyl-sn-glycero-3-phosphocholine (dic8pc), were selected as a bicellar model to study their phase behavior. over the whole range of dppc/dic8pc molar ratios (q) studied in this work, a lamellar crystalline phase (l-c') enriched in dppc was found to be the most stable phase at 5 degrees c, together with a dic8pc-enriched micelle phase. interestingly, a metastable phase, named the u phase in this work, was observed in the mixtures with a dppc/dic8pc molar ratio between 1 and 4. the metastable u phase was found to be lacking in long-range order in the direction of the bilayer surface normal, but bearing a different "crystalline phase-like" hydrocarbon chain packing mode, in comparison with the lamellar crystalline phase. the kinetic properties of the u phase were also studied in detail, and it was found that the phase acts as a precursor phase in the process of forming themost stable crystalline phase. this work deepens our understanding of lipid crystallization behavior, and is also a step forward towards a more detailed picture of the phase behavior of lipid mixtures composed of long-and short-chain lipids. |
| WOS关键词 | X-RAY-DIFFRACTION ; SOLID-STATE NMR ; HYDRATED DIPALMITOYLPHOSPHATIDYLCHOLINE BILAYERS ; NUCLEAR-MAGNETIC-RESONANCE ; METASTABLE SUBGEL PHASE ; MEMBRANE-PROTEINS ; SATURATED DIACYLPHOSPHATIDYLCHOLINES ; MODEL MEMBRANES ; BICELLES ; SPECTROSCOPY |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000393754100021 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2176843 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Yu, Zhi-Wu |
| 作者单位 | 1.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorous Chem & Chem Biol, Minist Educ, Beijing 100084, Peoples R China 2.Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China 3.Chinese Acad Sci, Beijing Synchrotron Radiat Facil, Inst High Energy Phys, Beijing 100049, Peoples R China 4.Qingdao Univ, Sch Chem & Chem Engn, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Hai-Yuan,Wu, Fu-Gen,Li, Zhi-Hong,et al. Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines[J]. Rsc advances,2017,7(10):5715-5724. |
| APA | Sun, Hai-Yuan,Wu, Fu-Gen,Li, Zhi-Hong,Deng, Geng,Zhou, Yu,&Yu, Zhi-Wu.(2017).Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines.Rsc advances,7(10),5715-5724. |
| MLA | Sun, Hai-Yuan,et al."Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines".Rsc advances 7.10(2017):5715-5724. |
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