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	Reverse fluorescence enhancement and colorimetric bimodal signal readout immunochromatography test strip for ultrasensitive large-scale screening and postoperative monitoring
	Yao, Yingyi1; Guo, Weisheng1,2; Zhang, Jian1; Wu, Yudong1; Fu, Weihua3; Liu, Tingting3; Wu, Xiaoli1; Wang, Hanjie1; Gong, Xiaoqun1; Liang, Xing-jie2
刊名	Acs applied materials & interfaces
	2016-09-07
卷号	8期号:35页码:22963-22970
关键词	Poct Reverse fluorescence enhancement icts (rficts) Carcinoembryonic antigen (cea) Bimodal signal readout Postoperative monitoring
ISSN号	1944-8244
DOI	10.1021/acsami.6b08445
通讯作者	Gong, xiaoqun(gongxiaoqun@tju.edu.cn) ; Chang, jin(jinchang@tju.edu.cn)
英文摘要	Ultrasensitive and quantitative fast screening of cancer biomarkers by immunochromatography test strip (icts) is still challenging in clinic. the gold nanoparticles (nps) based icts with colorimetric readout enables a quick spectrum screening but suffers from nonquantitative performance; although icts with fluorescence readout (ficts) allows quantitative detection, its sensitivity still deserves more efforts and attentions. in this work, by taking advantages of colorimetric icts and ficts, we described a reverse fluorescence enhancement icts (rficts) with bimodal signal readout for ultrasensitive and quantitative fast screening of carcinoembryonic antigen (cea). in the presence of target, gold nps aggregation in t line induced colorimetric readout, allowing on-the-spot spectrum screening in 10 min by naked eye. meanwhile, the reverse fluorescence enhancement signal enabled more accurately quantitative detection with better sensitivity (5.89 pg/ml for cea), which is more than 2 orders of magnitude lower than that of the conventional ficts. the accuracy and stability of the rficts were investigated with more than 100 clinical serum samples for large-scale screening. furthermore, this rficts also realized postoperative monitoring by detecting cea in a patient with colon cancer and comparing with ct imaging diagnosis. these results indicated this rficts is particularly suitable for point-of-care (poc) diagnostics in both resource-rich and resource-limited settings.
WOS关键词	POINT-OF-CARE ; LATERAL FLOW TEST ; GOLD NANOPARTICLES ; SENSITIVE DETECTION ; QUANTITATIVE DETECTION ; STAPHYLOCOCCUS-AUREUS ; ALPHA-FETOPROTEIN ; COLLOIDAL GOLD ; QUANTUM DOTS ; TEST KIT
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000382902800026
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176586
专题	高能物理研究所
通讯作者	Gong, Xiaoqun; Chang, Jin
作者单位	1.Tianjin Univ, Sch Mat Sci & Engn, Collaborat Innovat Ctr Chem Sci & Engn, Sch Life Sci,Tianjin Engn Ctr Micronano Biomat &, Tianjin 300072, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Lab Controllable Nanopharmaceut, Beijing 100190, Peoples R China 3.Tianjin Med Univ, Dept Gen Surg, Gen Hosp, Tianjin 300052, Peoples R China
推荐引用方式 GB/T 7714	Yao, Yingyi,Guo, Weisheng,Zhang, Jian,et al. Reverse fluorescence enhancement and colorimetric bimodal signal readout immunochromatography test strip for ultrasensitive large-scale screening and postoperative monitoring[J]. Acs applied materials & interfaces,2016,8(35):22963-22970.
APA	Yao, Yingyi.,Guo, Weisheng.,Zhang, Jian.,Wu, Yudong.,Fu, Weihua.,...&Chang, Jin.(2016).Reverse fluorescence enhancement and colorimetric bimodal signal readout immunochromatography test strip for ultrasensitive large-scale screening and postoperative monitoring.Acs applied materials & interfaces,8(35),22963-22970.
MLA	Yao, Yingyi,et al."Reverse fluorescence enhancement and colorimetric bimodal signal readout immunochromatography test strip for ultrasensitive large-scale screening and postoperative monitoring".Acs applied materials & interfaces 8.35(2016):22963-22970.

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