| High-throughput sample-to-answer detection of dna/rna in crude samples within functionalized micro-pipette tips | |
| Lu, Wenjing1,2,3; Wang, Jidong1,2; Wu, Qiong4; Sun, Jiashu1,2; Chen, Yiping1,2; Zhang, Lu1,2,3; Zheng, Chunsheng5; Gao, Wenna5; Liu, Yi4; Jiang, Xingyu1,2 | |
| 刊名 | Biosensors & bioelectronics
 |
| 2016-01-15 | |
| 卷号 | 75页码:28-33 |
| 关键词 | Nucleic acid test Dna Rna Sample-to-answer Crude samples |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2015.08.016 |
| 通讯作者 | Sun, jiashu(sunjs@nanoctr.cn) |
| 英文摘要 | We develop a micro-pipette tip-based nucleic acid test (mtnt) for high-throughput sample-to-answer detection of both dna and rna from crude samples including cells, bacteria, and solid plants, without the need of sample pretreatment and complex operation. mtnt consists of micro-pipette tips and embedded solid phase nucleic acid extraction membranes, and fully integrates the functions of nucleic acid extraction from crude samples, loop-mediated isothermal amplification (lamp) of nucleic acids, and visual readout of assays. the total assaying time for dna or rna from a variety of crude samples ranges from 90 to 160 min, the limit of detection (lod) of mtnt is 2 copies of plasmids containing the target nucleic acid fragments of ebola virus, and 8 cfu of escherichia coli carrying ebola virus-derived plasmids. mtnt can also detect ck-19 mrna from as few as 2 cancer cells without complicated procedures such as rna extraction and purification. we further demonstrate mtnt in a high-throughput format using an eight-channel pipette and a homemade mini-heater, with a maximum throughput of 40 samples. compared with other point-of-care (poc) nucleic acid tests (nat), mtnt could assay both dna and rna directly from liquid (cells/bacteria/blood) or solid (plant) samples in a straightforward, sensitive, high-throughput, and containment-free manner, suggesting a considerable promise for low-cost and poc nat in remote areas. (c) 2015 elsevier b.v. all rights reserved. |
| WOS关键词 | MEDIATED ISOTHERMAL AMPLIFICATION ; QUANTITATIVE DETECTION ; DIAGNOSING INFECTIONS ; MICROFLUIDIC SYSTEM ; DEVELOPING-WORLD ; NUCLEIC-ACIDS ; EMULSION PCR ; PATHOGENS ; BACTERIA ; MICROCAPILLARY |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| WOS记录号 | WOS:000362862000005 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2176493 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Sun, Jiashu |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sinodanish Ctr Educ & Res, Sinodanish Coll UCAS, Beijing 100190, Peoples R China 4.Acad Mil Med Sci, Affiliated Hosp, Hosp 307, Beijing 100071, Peoples R China 5.Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Wenjing,Wang, Jidong,Wu, Qiong,et al. High-throughput sample-to-answer detection of dna/rna in crude samples within functionalized micro-pipette tips[J]. Biosensors & bioelectronics,2016,75:28-33. |
| APA | Lu, Wenjing.,Wang, Jidong.,Wu, Qiong.,Sun, Jiashu.,Chen, Yiping.,...&Jiang, Xingyu.(2016).High-throughput sample-to-answer detection of dna/rna in crude samples within functionalized micro-pipette tips.Biosensors & bioelectronics,75,28-33. |
| MLA | Lu, Wenjing,et al."High-throughput sample-to-answer detection of dna/rna in crude samples within functionalized micro-pipette tips".Biosensors & bioelectronics 75(2016):28-33. |
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