| Peptide-conjugated gold nanoprobe: intrinsic nanozyme-linked immunsorbant assay of integrin expression level on cell membrane | |
| Gao, Liang; Liu, Meiqing; Ma, Guifu; Wang, Yaling; Zhao, Lina; Yuan, Qing; Gao, Fuping; Liu, Ru; Zhai, Jiao; Chai, Zhifang | |
| 刊名 | Acs nano
 |
| 2015-11-01 | |
| 卷号 | 9期号:11页码:10979-10990 |
| 关键词 | Nanozyme Enzyme-linked immunosorbent assay Gold nanoparticles Membrane protein quantification Two-photon imaging |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.5b04261 |
| 通讯作者 | Gao, xueyun(gaoxy@ihep.ac.cn) |
| 英文摘要 | Precisely quantifying the membrane protein expression level on cell surfaces is of vital importance for early cancer diagnosis and efficient treatment. we demonstrate that gold nanoparticle bioconjugated by a rationally designed peptide as nanoprobe possesses selective labeling and accurate quantification capacity of integrin gpilb/iiia on the human erythroleukemia cell line. through selective recognition and marking of integrin, two-photon photoluminescence of the nanoprobe is exploited for direct observation of protein spatial distribution on cell membrane. more importantly, utilizing intrinsic enzyme-like catalysis property of the nanoprobe, the expression level of integrin on human erythroleukemia cells can be quantitatively counted in an amplified and reliable colorimetric assay without cell lysis and protein extraction process. in addition, the analysis of the correlation between the gold nanoparticle and the membrane protein via relevant inductively coupled plasma mass spectrometry measurement verifies the reliability of the new analytical method. it is anticipated that this facile and efficient strategy holds a great promise for a rapid, precise, and reliable quantification of interested functional membrane proteins on the cell surface. |
| WOS关键词 | PEROXIDASE-LIKE ACTIVITY ; ARTIFICIAL ENZYMES ; NANOPARTICLES ; NANOCLUSTERS ; GENERATION ; PHOTOLUMINESCENCE ; NANOMATERIALS ; SPECTROSCOPY ; CHALLENGES ; STRATEGIES |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000365464800046 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2176440 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Gao, Xueyun |
| 作者单位 | Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Liang,Liu, Meiqing,Ma, Guifu,et al. Peptide-conjugated gold nanoprobe: intrinsic nanozyme-linked immunsorbant assay of integrin expression level on cell membrane[J]. Acs nano,2015,9(11):10979-10990. |
| APA | Gao, Liang.,Liu, Meiqing.,Ma, Guifu.,Wang, Yaling.,Zhao, Lina.,...&Gao, Xueyun.(2015).Peptide-conjugated gold nanoprobe: intrinsic nanozyme-linked immunsorbant assay of integrin expression level on cell membrane.Acs nano,9(11),10979-10990. |
| MLA | Gao, Liang,et al."Peptide-conjugated gold nanoprobe: intrinsic nanozyme-linked immunsorbant assay of integrin expression level on cell membrane".Acs nano 9.11(2015):10979-10990. |
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